Document Detail


MERRF: Clinical features, muscle biopsy and molecular genetics in Brazilian patients.
MedLine Citation:
PMID:  21303704     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Myoclonic epilepsy with ragged red fibers (MERRF) is a mitochondrial disease that is characterized by myoclonic epilepsy with ragged red fibers (RRF) in muscle biopsies. The aim of this study was to analyze Brazilian patients with MERRF. Six patients with MERRF were studied and correlations between clinical findings, laboratory data, electrophysiology, histology and molecular features were examined. We found that blood lactate was increased in four patients. Electroencephalogram studies revealed generalized epileptiform discharges in five patients and generalized photoparoxysmal responses during intermittent photic stimulation in two patients. Muscle biopsies showed RRF in all patients using modified Gomori-trichrome and succinate dehydrogenase stains. Cytochrome c oxidase (COX) stain analysis indicated deficient activity in five patients and subsarcolemmal accumulation in one patient. Molecular analysis of the tRNA(Lys) gene with PCR/RFLP and direct sequencing showed the A8344G mutation of mtDNA in five patients. The presence of RRFs and COX deficiencies in muscle biopsies often confirmed the MERRF diagnosis. We conclude that molecular analysis of the tRNA(Lys) gene is an important criterion to help confirm the MERRF diagnosis. Furthermore, based on the findings of this study, we suggest a revision of the main characteristics of this disease.
Authors:
Paulo José Lorenzoni; Rosana H Scola; Cláudia S Kamoi Kay; Raquel C Arndt; Carlos E Silvado; Lineu C Werneck
Related Documents :
14502264 - Mortality in severe, non aggressively treated adult autoimmune thrombocytopenia.
9640794 - Hemorrhagic and thrombotic complications in patients with myeloproliferative diseases.
6828674 - Management of massive hemoptysis by bronchial artery embolization.
9107384 - Quantitative systemic and local evaluation of the antiviral effect of ganciclovir and f...
11921694 - The risk of radiocontrast nephropathy in patients with cirrhosis.
8523614 - Complications of peripheral arteriography: a new system to identify patients at increas...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-5
Journal Detail:
Title:  Mitochondrion     Volume:  -     ISSN:  1872-8278     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100968751     Medline TA:  Mitochondrion     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010. Published by Elsevier B.V.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  There is no evidence that mitochondria are the main source of reactive oxygen species in mammalian c...
Next Document:  Recommendations for the management of mixed Cryoglobulinemia syndrome in Hepatitis C virus-infected ...