Document Detail

MEK1 and MEK2 differentially regulate human insulin- and insulin glargine-induced human bladder cancer T24 cell proliferation.
MedLine Citation:
PMID:  23217386     Owner:  NLM     Status:  In-Data-Review    
BACKGROUND: Increased risk of bladder cancer has been reported in diabetic patients. This study was to investigate the roles of mitogen-activated protein kinase kinase (MEK) 1 and 2 in the regulation of human insulin- and insulin glargine-induced proliferation of human bladder cancer T24 cells.
METHODS: In the absence or presence of a selective inhibitor for MEK1 (PD98059) or a specific siRNA for MEK2 (siMEK2), with or without addition of insulin or glargine, T24 cell proliferation was evaluated by cell counting kit (CCK)-8 assay. Protein expression of MEK2, phosphorylation of ERK1/2 and Akt was analyzed by Western blotting.
RESULTS: T24 cell proliferation was promoted by PD98059 at 5 - 20 µmol/L, inhibited by siMEK2 at 25 - 100 nmol/L. PD98059 and siMEK2 remarkably reduced phosphorylated ERK1/2. Insulin- and glargine-induced T24 cell proliferation was enhanced by PD98059, suppressed while not blocked by siMEK2. Insulin- and glargine-induced ERK1/2 activation was blocked by PD98059 or siMEK2 treatment, whereas activation of Akt was not affected.
CONCLUSION: MEK1 inhibits while MEK2 contributes to normal and human insulin- and insulin glargine-induced human bladder cancer T24 cell proliferation.
Shan-Ying Liu; Ying Liang; Tian-Xin Lin; Fang Su; Wei-Wen Liang; Heemann Uwe; Yan Li
Related Documents :
17064806 - Vascular phenotype and subclinical inflammation in diabetic asian indians without overt...
23489776 - Selenium supplementation has no effect on serum glucose levels in men at high risk for ...
18760286 - Association of glycated albumin, but not glycated hemoglobin, with peripheral vascular ...
10371376 - Moderate dietary salt restriction increases vascular and systemic insulin resistance.
25010776 - Acanthosis nigricans, vitamin d, and insulin resistance in obese children and adolescents.
6764506 - Continuous insulin infusion in hyperglycemic, very low birth weight infants.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  125     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  4197-201     Citation Subset:  IM    
Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Risk factors and pain status due to diabetic neuropathy in chronic long-term diabetic patients in a ...
Next Document:  MicroRNA-34a contributes to the protective effects of glucagon-like peptide-1 against lipotoxicity i...