Document Detail


MEK-ERK signaling in adult newt retinal pigment epithelium cells is strengthened immediately after surgical induction of retinal regeneration.
MedLine Citation:
PMID:  22743657     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Adult newt retinal pigment epithelium (RPE) cells are mitotically quiescent in the physiological condition, but upon a traumatic injury of the neural retina (NR) they re-enter the cell-cycle and eventually regenerate the missing NR. Here, to understand the mechanism underlying the cell-cycle re-entry of RPE cells following NR injury, we first investigated changes in MEK-ERK signaling activity in RPE cells upon removal of the NR (retinectomy) from the eye of living animals, and found that ERK-mediated signaling activity is elevated quickly (in 30min) upon retinectomy. In addition, we found, in in vitro analyses, that immediate early activation of MEK-ERK signaling may occur in RPE cells upon NR injury, intensifying the MEK-ERK signaling itself through up-regulation of the expression of constituent molecules in the pathway, and that 1-h blockade of such early MEK-ERK signaling interferes with the cell-cycle re-entry, which occurs 5-10 days later. Together, these results provide us with insight that elevation of MEK-ERK signaling activity upon NR injury may be a key process for mitotically quiescent RPE cells to re-enter the cell-cycle, leading to retinal regeneration.
Authors:
Aki Mizuno; Hirofumi Yasumuro; Taro Yoshikawa; Wataru Inami; Chikafumi Chiba
Related Documents :
18253947 - Cell (a549)-particle (jasada bhasma) interactions using raman spectroscopy.
22297397 - L-n-iminoethyl-lysine after experimental brain trauma attenuates cellular proliferation...
8803797 - Differential expression of the 45 kda protein (actin) during the dimorphic transition o...
18047657 - Metabolic signature of breast cancer cell line mcf-7: profiling of modified nucleosides...
20585557 - Fungal cell gigantism during mammalian infection.
24195507 - Cyclin-dependent kinase 1 inhibitor ro3306 promotes mitotic slippage in paclitaxel-trea...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-25
Journal Detail:
Title:  Neuroscience letters     Volume:  -     ISSN:  1872-7972     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Affiliation:
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki 305-8572 Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The Effect of Early Attention Allocation on Location-based Attention toward a Later Threat: An ERP S...
Next Document:  Analysis of ATP13A2 in large neurodegeneration with brain iron accumulation (NBIA) and dystonia-park...