| MEC3, MEC1, and DDC2 are essential components of a telomere checkpoint pathway required for cell cycle arrest during senescence in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 12181334 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When telomerase is absent and/or telomeres become critically short, cells undergo a progressive decline in viability termed senescence. The telomere checkpoint model predicts that cells will respond to a damaged or critically short telomere by transiently arresting and activating repair of the telomere. We examined the senescence of telomerase-deficient Saccharomyces cerevisiae at the cellular level to ask if the loss of telomerase activity triggers a checkpoint response. As telomerase-deficient mutants were serially subcultured, cells exhibited a progressive decline in average growth rate and an increase in the number of cells delayed in the G2/M stage of the cell cycle. MEC3, MEC1, and DDC2, genes important for the DNA damage checkpoint response, were required for the cell cycle delay in telomerase-deficient cells. In contrast, TEL1, RAD9, and RAD53, genes also required for the DNA damage checkpoint response, were not required for the G2/M delay in telomerase-deficient cells. We propose that the telomere checkpoint is distinct from the DNA damage checkpoint and requires a specific set of gene products to delay the cell cycle and presumably to activate telomerase and/or other telomere repair activities. |
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Authors:
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Shinichiro Enomoto; Lynn Glowczewski; Judith Berman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular biology of the cell Volume: 13 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-15 Completed Date: 2003-04-09 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2626-38 Citation Subset: IM |
Affiliation:
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Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul 55108, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Signal Transducing Cell Cycle / physiology* Cell Cycle Proteins / genetics, metabolism* DNA Damage Fungal Proteins / metabolism Genes, cdc Intracellular Signaling Peptides and Proteins Phosphoproteins / genetics, metabolism* Protein-Serine-Threonine Kinases / metabolism Saccharomyces cerevisiae / cytology, physiology* Saccharomyces cerevisiae Proteins / genetics, metabolism* Telomerase / metabolism Telomere / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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F32 GM 63352/GM/NIGMS NIH HHS; GM 38626/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/Cell Cycle Proteins; 0/Fungal Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/LCD1 protein, S cerevisiae; 0/MEC3 protein, S cerevisiae; 0/Phosphoproteins; 0/Saccharomyces cerevisiae Proteins; 139691-42-2/rad9 protein; EC 2.7.1.-/RAD53 protein, S cerevisiae; EC 2.7.11.1/MEC1 protein, S cerevisiae; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/TEL1 protein, S cerevisiae; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
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