Document Detail


MDM2 interacts with and downregulates a sarcomeric protein, TCAP.
MedLine Citation:
PMID:  16678796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent reports have shown that MDM2 may attenuate hypertrophy of cardiac myocytes. However, mechanism of MDM2 involving in this process is unclear. In this study, we identified a novel specific MDM2-binding protein TCAP by the yeast two-hybrid screen. It was validated by GST pull-down and co-immunoprecipitation assays. Confocal analysis showed that MDM2 and TCAP co-localized in the nucleus, and elevated MDM2 expression could alter the subcellular localization of TCAP. Notably, MDM2 downregulated the protein level of TCAP through the proteasomal pathway, and this downregulation was inhibited by p14(ARF). In addition, our results suggested that the degradation of TCAP by MDM2 was through the ubiquitin-independent pathway. Given that TCAP is a key component involving in the cardiac hypertrophy, the degradation of TCAP by MDM2 might be connected with the roles of MDM2 in cardiac hypertrophy. Further investigation will focus on the biological significance of MDM2-TCAP interaction in cardiac hypertrophy.
Authors:
Li-Feng Tian; Hui-Yan Li; Bao-Feng Jin; Xin Pan; Jiang-Hong Man; Pei-Jing Zhang; Wei-Hua Li; Bing Liang; Hui Liu; Jie Zhao; Wei-Li Gong; Tao Zhou; Xue-Min Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-02
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  345     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-17     Completed Date:  2006-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-61     Citation Subset:  IM    
Affiliation:
National Center of Biomedical Analysis, Beijing 100850, China.
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MeSH Terms
Descriptor/Qualifier:
Down-Regulation
Muscle Proteins / chemistry,  metabolism*
Protein Binding
Protein Interaction Mapping
Proto-Oncogene Proteins c-mdm2 / chemistry,  metabolism*
Sarcomeres / chemistry,  metabolism*
Subcellular Fractions / chemistry,  metabolism*
Chemical
Reg. No./Substance:
0/Muscle Proteins; 0/Tcap protein, mouse; EC 6.3.2.19/Mdm2 protein, mouse; EC 6.3.2.19/Proto-Oncogene Proteins c-mdm2

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