Document Detail


MARTX of Vibrio vulnificus biotype 2 is a virulence and survival factor.
MedLine Citation:
PMID:  22943291     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Vibrio vulnificus biotype 2 is a polyphyletic group whose virulence for fish relies on a plasmid. This plasmid contains an rtxA gene duplicated in the small chromosome that encodes a MARTX (Multifunctional, Autoprocessing Repeats-in-Toxin) unique within the species in domain structure (MARTX type III). To discover the role of this toxin in the fitness of this biotype in the fish-farming environment, single- and double-knockout mutants were isolated from a zoonotic strain and analysed in a series of in vivo and in vitro experiments with eel, fish cell lines and amoebae isolated from gills. Mice, murine and human cell lines were also assayed for comparative purposes. The results suggest that MARTX type III is involved in the lysis of a wide range of eukaryotic cells, including the amoebae, erythrocytes, epithelial cells and phagocytes after bacterium-cell contact. In fish, MARTX type III may act as a toxin involved in the onset of septic shock, while in mice it may promote bacterial colonization by preventing phagocytosis of bacterial cells. Moreover, this toxin could protect bacteria from predation by amoebae, which would increase bacterial survival outside the host and would explain the fitness of this biotype in the fish-farming environment.
Authors:
Chung-Te Lee; David Pajuelo; Amparo Llorens; Yi-Hsuan Chen; José M Leiro; Francesc Padrós; Lien-I Hor; Carmen Amaro
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-30
Journal Detail:
Title:  Environmental microbiology     Volume:  -     ISSN:  1462-2920     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-9-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan, 701, Taiwan.
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