| MAPKs and Mst1/Caspase-3 pathways contribute to H2B phosphorylation during UVB-induced apoptosis. | |
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MedLine Citation:
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PMID: 20602268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis is a highly coordinated or programmed cell suicide mechanism in eukaryotes. Histone modification is associated with nuclear events in apoptotic cells. Specifically H2B phosphorylation at serine 14 (Ser14) catalyzed by Mst1 kinase has been linked to chromatin condensation during apoptosis. We report that activation of MAPKs (ERK1/2, JNK1/2 and p38) together with Mst1 and caspase-3 is required for phosphorylation of H2B (Ser14) during ultraviolet B light (UVB)-induced apoptosis. UVB can trigger activation of MAPKs and induce H2B phosphorylation at Ser14 but not acetylation in a time-dependent manner. Inhibition of ERK1/2, JNK1/2 or p38 activity blocked H2B phosphorylation (Ser14). Furthermore, caspase-3 was activated by UVB to regulate Mst1 activity, which phosphorylates H2B at Ser14, leading to chromatin condensation. Full inhibition of caspase-3 activity reduced Mst1 activation and partially inhibited H2B phosphorylation (Ser14), but ERK1/2, JNK1/2 and p38 activities were not affected. Taken together, these data revealed that H2B phosphorylation is regulated by both MAPKs and caspase-3/Mst1 pathways during UVB-induced apoptosis. |
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Authors:
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ChengRong Lu; Ying Shi; Yuan Luo; LianNing Duan; Yong Hou; HongBo Hu; Zhe Wang; PeiDe Xiang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-03 |
Journal Detail:
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Title: Science China. Life sciences Volume: 53 ISSN: 1869-1889 ISO Abbreviation: Sci China Life Sci Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-07-05 Completed Date: 2010-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101529880 Medline TA: Sci China Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 663-8 Citation Subset: IM |
Affiliation:
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Aviation Medicine Research Laborabory, General Hospital of Air Force, Beijing, 100142, China. luchengrong@263.net |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Apoptosis / radiation effects* Blotting, Western Caspase 3 / metabolism* Cells, Cultured Flow Cytometry Fluorescent Antibody Technique Histones / metabolism* Humans Mitogen-Activated Protein Kinases / metabolism* Phosphorylation Protein-Serine-Threonine Kinases / metabolism* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Histones; EC 2.7.1.-/STK4 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.4.22.-/Caspase 3 |
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