| MALDI imaging mass spectrometry of phospholipids in the mouse lung. | |
| | |
MedLine Citation:
|
PMID: 21508254 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are polyunsaturated fatty acids (PUFA) that are present in phospholipids in cell membranes. In this study, matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) was used to determine the localization of arachidonate- and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology. |
| | |
Authors:
|
Karin A Zemski Berry; Bilan Li; Susan D Reynolds; Robert M Barkley; Miguel A Gijon; Joseph A Hankin; Peter M Henson; Robert C Murphy |
Related Documents
:
|
6845564 - Separate examinations on core and shell of urinary calculi. 2214614 - Acute uric acid nephropathy in two gouty patients with moderate hyperuricemia and high ... 7056604 - The effect of prednisone and azathioprine (imuran) on renal excretion of uric acid. 19112124 - Characterization of a degenerative cardiomyopathy associated with domoic acid toxicity ... 8659944 - Obagi's modified trichloroacetic acid (tca)-controlled variable-depth peel: a study of ... 1154054 - Anesthesia and the porphyrias. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-20 |
Journal Detail:
|
Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-4-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
University of Colorado Denver, United States; |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ethical Reflections: Examining Reflexivity Through the Narrative Paradigm.
Next Document: Filipin recognizes both GM1 and cholesterol in GM1 gangliosidosis mouse brain.