Document Detail


MALDI imaging MS of phospholipids in the mouse lung.
MedLine Citation:
PMID:  21508254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate- and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species, and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology.
Authors:
Karin A Zemski Berry; Bilan Li; Susan D Reynolds; Robert M Barkley; Miguel A Gijón; Joseph A Hankin; Peter M Henson; Robert C Murphy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-04-20
Journal Detail:
Title:  Journal of lipid research     Volume:  52     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-18     Completed Date:  2011-11-08     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1551-60     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Colorado Denver, 12801 East 17 Avenue, Mail Stop 8303, Aurora, CO 80045, USA. Robert.Murphy@ucdenver.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / analysis,  metabolism
Blood Vessels / chemistry*,  metabolism
Docosahexaenoic Acids / analysis,  metabolism
Fluorescent Antibody Technique
Lipid Metabolism*
Lung / chemistry*,  metabolism
Mice
Mice, Inbred C57BL
Phospholipids / analysis*,  metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Sphingomyelins / analysis*,  metabolism
Grant Support
ID/Acronym/Agency:
GM069338/GM/NIGMS NIH HHS; HL-25785/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Phospholipids; 0/Sphingomyelins; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid
Comments/Corrections

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