Document Detail

MALDI Mass Spectrometry for Nucleic Acid Analysis.
MedLine Citation:
PMID:  23019094     Owner:  NLM     Status:  Publisher    
With the discovery of several matrices which enable the ionization of DNA and RNA, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become a powerful platform for the study of nucleic acid sequence changes (e.g., mutations, single nucleotide polymorphisms (SNPs), insertion/deletion, alternative splicing, etc.), amount changes (e.g., copy number variation, gene expression, allele expression, etc.), as well as modifications (e.g., methylation of genomic DNA, post transcriptional modification of tRNAs and rRNAs). Two major strategies have been employed to characterize these changes. Primer extension reactions are designed for genotyping of known polymorphic sites and determining the levels of gene or allele expressions. Base-specific cleavage reactions are used for discovery of unknown polymorphisms and characterization of modifications. These two assays usually generate nucleic acid fragments less than 30 bases in length, which is the ideal mass range for MALDI-MS. Here we review the basic concepts of these assays, sample analysis techniques, and their applications published in recent years.
Xiang Gao; Boon-Huan Tan; Richard J Sugrue; Kai Tang
Related Documents :
23422674 - Disease mutations in the ryanodine receptor n-terminal region couple to a mobile inters...
23077214 - 22q11 gene dosage establishes an adaptive range for sonic hedgehog and retinoic acid si...
17777604 - A correlation between gametophytic and sporophytic characteristics in zea mays l.
23599814 - The role of androgen receptor activity mediated by the cag repeat polymorphism in the p...
22435454 - Meta-analysis of the cystatin c(cst3) gene g73a polymorphism and susceptibility to alzh...
22727704 - The evolution of low mutation rates in experimental mutator populations of saccharomyce...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-28
Journal Detail:
Title:  Topics in current chemistry     Volume:  -     ISSN:  0340-1022     ISO Abbreviation:  Top Curr Chem     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0432204     Medline TA:  Top Curr Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Robust estimation of causal effects of binary treatments in unconfounded studies with dichotomous ou...
Next Document:  Chirality and Protein Biosynthesis.