| M1-activated macrophages migration, a marker of aortic atheroma progression: a preclinical MRI study in mice. | |
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MedLine Citation:
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PMID: 20375846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: M1-activated Macrophages (M1M) play a major role in atherosclerotic lesions of aortic arch, promoting proinflammatory response. In vivo trafficking of M1M in aortic plaques is therefore critical. METHODS: M1M from bone marrow cell culture were magnetically labeled, using iron nanoparticles, intravenously injected and followed up with 3 day magnetic resonance imaging (MRI) in mice developing macrophage-laden atheroma (ApoE2 knock-in mice). M1M recruitment in aortic arch lesions was assessed both by MRI and histology. RESULTS: In all ApoE2 knock-in mice injected with labeled cells, high resolution MRI showed localized signal loss regions in the thickened aortic wall, with a maximal effect at day 2 (-34% +/- 7.3% P < 0.001 compared with baseline). This was confirmed with Prussian blue (iron) staining and corresponded to M1M (Major Histo-compatibility Complex II positive). Clear different intraplaque and adventitial dynamic distribution profiles of labeled cells were observed during the 3 days. CONCLUSION: M1M dynamic MRI is a promising marker to noninvasively assess the macrophage trafficking underlying aortic arch plaque progression. |
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Authors:
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Amine Bessaad; Monica Sigovan; Hasan Alsaid; Geneviève De Souza; Nicolas Provost; Zouher Majd; Christine Menager; Jèrôme Honnorat; Florence Lagarde; Norbert Nighoghossian; Serge Nataf; Emmanuelle Canet-Soulas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative radiology Volume: 45 ISSN: 1536-0210 ISO Abbreviation: Invest Radiol Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-15 Completed Date: 2010-07-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045377 Medline TA: Invest Radiol Country: United States |
Other Details:
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Languages: eng Pagination: 262-9 Citation Subset: IM |
Affiliation:
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CREATIS-LRMN, UMR CNRS 5220, U630 INSERM, University Lyon 1, Villeurbanne Cedex, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aortic Diseases / diagnosis* Apolipoprotein E2 / genetics Arteriosclerosis / diagnosis* Biological Markers Gene Knock-In Techniques Macrophage Activation / physiology* Macrophages / physiology Magnetic Resonance Imaging* Mice Mice, Transgenic |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein E2; 0/Biological Markers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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