| M-cadherin-inhibited phosphorylation of ß-catenin augments differentiation of mouse myoblasts. | |
| | |
MedLine Citation:
|
PMID: 23138569 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
β-Catenin is essential for muscle development because it regulates both cadherin-mediated cell-cell adhesion and canonical Wingless and Int1 (Wnt) signaling. The phosphorylation of β-catenin by glycogen synthase kinase-3β (GSK-3β) at serine31/37/threonine41 regulates its stability and its role in canonical Wnt signaling. In this study, we have investigated whether the N-terminal phosphorylation of β-catenin is regulated by M-cadherin, and whether this regulation mediates the role of M-cadherin in myogenic differentiation. Our data show that the knockdown of M-cadherin expression by RNA interference (RNAi) in C2C12 myoblasts significantly increases the phosphorylation of β-catenin at Ser33/37/Thr41 and decreases the protein abundance of ser37/thr41-unphosphorylated active β-catenin. Furthermore, M-cadherin RNAi promotes TCF/LEF transcription activity but also blunts the initiation of the myogenic progress by Wnt pathway activator lithium chloride or Wnt-3a treatment. Knockdown of β-catenin expression by RNAi decreases myogenic induction in myoblasts. Forced expression of a phosphorylation-resistant β-catenin plasmid (S33Y-β-catenin) fails to enhance myogenic differentiation, but it partially rescues C2C12 cells from M-cadherin RNAi-induced apoptosis. These data show, for the first time, that M-cadherin-mediated signaling attenuates β-catenin phosphorylation at Ser31/37/Thr41 by GSK-3β, and that this regulation has a positive effect on myogenic differentiation induced by canonical Wnt signaling. |
| | |
Authors:
|
Yan Wang; Junaith S Mohamed; Stephen E Alway |
Related Documents
:
|
1954249 - Cytidine diphosphate diglyceride analogs of antiretroviral dideoxynucleosides: evidence... 23637019 - Inhibition of ck2 binding to bmpria induces c2c12 differentiation into osteoblasts and ... 23275879 - A targeted in vivo rnai screen reveals deubiquitinases as new regulators of notch signa... 21474569 - Rho-kinase inhibition by fasudil attenuates cyclosporine-induced kidney injury. 21252119 - Atra activates and pdgf-bb represses the sm22α promoter through klf4 binding to, or di... 6268279 - Preliminary studies of the uridine kinase activity of human colorectal adenocarcinomas. 19747539 - Naphtho[1,2-b]furan-4,5-dione induces apoptosis and s-phase arrest of mda-mb-231 cells ... 19717209 - Potentiation of paclitaxel-induced apoptosis by galectin-13 overexpression via activati... 23496219 - Non-atp-competitive kinase inhibitors - enhancing selectivity through new inhibition st... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
|
Title: Cell and tissue research Volume: - ISSN: 1432-0878 ISO Abbreviation: Cell Tissue Res. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratory of Muscle Biology and Sarcopenia, Department of Exercise Physiology, and Center for Cardiovascular and Respiratory Sciences, Robert C. Byrd Health Sciences Center, West Virginia University School of Medicine, Morgantown, WV 26506, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Connexin 26 facilitates gastrointestinal bacterial infection in vitro.
Next Document: Metabolic preconditioning of mammalian cells: mimetic agents for hypoxia lack fidelity in promoting ...