Document Detail


M-RIP, a novel target of JNK signaling and a requirement for human cancer cell invasion.
MedLine Citation:
PMID:  18636174     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell motility is involved in physiological and pathological processes such as the invasion and migration of cells. c-Jun N-terminal kinase (JNK) cascades are involved in the invasion and metastasis of cancer cells. However, little is known about the downstream signaling of JNK. In the present study, we used small interfering RNA (siRNA) directed against JNK1 to reduce its expression. We used microarray techniques to compare the gene expression profiles of epidermal growth factor (EGF)-stimulated HeLa cells with and without JNK1 siRNA treatment. We identified a JNK1 target gene, myosin phosphatase-Rho interacting protein (M-RIP). RNA interference-mediated inhibition of JNK1 strongly inhibited M-RIP mRNA expression induced by EGF, as well as the invasion of HeLa cells. In addition, M-RIP siRNA-treated cells showed significantly reduced invasive activity. Thus, a functional analysis of JNK1 and M-RIP with RNA interference reveals a critical role for this cascade in the invasive behavior of cancer cells.
Authors:
Ryoko Ono; Junji Matsuoka; Tomoki Yamatsuji; Yoshio Naomoto; Noriaki Tanaka; Hideki Matsui; Masayuki Matsushita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  22     ISSN:  1107-3756     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-18     Completed Date:  2008-09-18     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  199-203     Citation Subset:  IM    
Affiliation:
Department of Gastroenterological Surgery, Graduate School of Medicine and Dentistry, Okayama University, Okayama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / genetics,  metabolism*
Cell Movement / physiology*
Epidermal Growth Factor / metabolism
Eukaryotic Initiation Factor-4E / metabolism
Focal Adhesion Kinase 1 / genetics,  metabolism
Gene Expression Profiling
HeLa Cells
Humans
Mitogen-Activated Protein Kinase 8 / genetics,  metabolism*
Neoplasm Invasiveness*
Neoplasms / genetics,  metabolism*,  pathology*
RNA, Small Interfering / genetics,  metabolism
Signal Transduction / physiology*
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Eukaryotic Initiation Factor-4E; 0/M-RIP protein, human; 0/RNA, Small Interfering; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.2/PTK2 protein, human; EC 2.7.11.24/Mitogen-Activated Protein Kinase 8

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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