Document Detail

M and N proteins of SARS coronavirus induce apoptosis in HPF cells.
MedLine Citation:
PMID:  16845612     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: SARS-associated coronavirus (SARS-CoV) induced cell apoptosis and its structural proteins may play a role in this process. OBJECTIVES: To determine whether the structural proteins M and N of SARS-CoV induce apoptosis. STUDY DESIGN: We investigated human pulmonary fibroblast (HPF) cells, were transfected with plasmids containing the M or N gene, by TdT-mediated dUTP nick end labeling (TUNEL), Hoechst 33342 staining for nuclei, and observation of morphology. RESULTS: We found that in the absence of serum about 16.34% of cells transfected by pcDNA3.1-M and 21.72% of N-transfected cells showed typical apoptotic characteristics, significantly different from mock-transfected cells (only 6.23%, p<0.01). Furthermore, the cells that were co-transfected with M and N proteins showed more obvious phenomena of cell death (about 36.03%). There was a statistical significance between M-transfected cells and co-transfected cells (p<0.01), and a remarkable difference between N-transfected cells and co-transfected cells (p<0.01). CONCLUSIONS: The results show that M and N proteins of SARS-CoV can induce apoptosis of HPF cells. Co-transfection of M and N enhances the induction of apoptosis by M or N alone, which also suggests that the structural proteins of SARS-CoV may play an important role not only in the process of invasion but also in the pathogenetic process in cells.
Gang Zhao; Shu-Qun Shi; Ying Yang; Jing-Pian Peng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-16
Journal Detail:
Title:  Cell biology and toxicology     Volume:  22     ISSN:  0742-2091     ISO Abbreviation:  Cell Biol. Toxicol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-09     Completed Date:  2007-06-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506639     Medline TA:  Cell Biol Toxicol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  313-22     Citation Subset:  IM    
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100080, China.
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MeSH Terms
Blotting, Western
Cells, Cultured
Fibroblasts / cytology*
Genetic Vectors
In Situ Nick-End Labeling
Lung / cytology*
Nucleocapsid Proteins / metabolism*
SARS Virus*
Severe Acute Respiratory Syndrome
Viral Matrix Proteins / metabolism*
Reg. No./Substance:
0/Benzimidazoles; 0/Nucleocapsid Proteins; 0/Viral Matrix Proteins; 0/nucleocapsid protein, Coronavirus; 108502-71-2/M protein, Coronavirus; 23491-52-3/HOE 33342

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