Document Detail

Lytic phages obscure the cost of antibiotic resistance in Escherichia coli.
MedLine Citation:
PMID:  25268496     Owner:  NLM     Status:  Publisher    
The long-term persistence of antibiotic-resistant bacteria depends on their fitness relative to other genotypes in the absence of drugs. Outside the laboratory, viruses that parasitize bacteria (phages) are ubiquitous, but costs of antibiotic resistance are typically studied in phage-free experimental conditions. We used a mathematical model and experiments with Escherichia coli to show that lytic phages strongly affect the incidence of antibiotic resistance in drug-free conditions. Under phage parasitism, the likelihood that antibiotic-resistant genetic backgrounds spread depends on their initial frequency, mutation rate and intrinsic growth rate relative to drug-susceptible genotypes, because these parameters determine relative rates of phage-resistance evolution on different genetic backgrounds. Moreover, the average cost of antibiotic resistance in terms of intrinsic growth in the antibiotic-free experimental environment was small relative to the benefits of an increased mutation rate in the presence of phages. This is consistent with our theoretical work indicating that, under phage selection, typical costs of antibiotic resistance can be outweighed by realistic increases in mutability if drug resistance and hypermutability are genetically linked, as is frequently observed in clinical isolates. This suggests the long-term distribution of antibiotic resistance depends on the relative rates at which different lineages adapt to other types of selection, which in the case of phage parasitism is probably extremely common, as well as costs of resistance inferred by classical in vitro methods.The ISME Journal advance online publication, 30 September 2014; doi:10.1038/ismej.2014.176.
Samuel J Tazzyman; Alex R Hall
Related Documents :
25469776 - A new oligonucleotide microarray for detection of pathogenic and non-pathogenic legione...
16504506 - Synthesis of a potent 5'-methylthioadenosine/s-adenosylhomocysteine (mtan) inhibitor.
25363146 - Multicentric study in five african countries of antibiotic susceptibility for three mai...
25300356 - Virulence factors genes in enterococci isolated from beavers (castor fiber).
4919756 - Genetic control of the secondary modification of deoxyribonucleic acid in escherichia c...
23997786 - Mechanisms of reduced susceptibility to ciprofloxacin in escherichia coli isolates from...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-30
Journal Detail:
Title:  The ISME journal     Volume:  -     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-30     Completed Date:  -     Revised Date:  2014-10-1    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Impact of Combined Clenbuterol and Metoprolol Therapy on Reverse Remodelling during Mechanical Unloa...
Next Document:  The Mycotoxin Deoxynivalenol Predisposes for the Development of Clostridium perfringens-Induced Necr...