Document Detail

Lysosomes in lymphoid tissue. II. Intracellular distribution of acid hydrolases.
Jump to Full Text
MedLine Citation:
PMID:  10976226     Owner:  NLM     Status:  MEDLINE    
Differential centrifugation and density gradient isopycnic centrifugation have been used to fractionate homogenates of rat spleen and, in a few experiments, of rat thymus and cervical lymph nodes. The fractions have been analyzed for proteins, DNA, RNA, cytochrome oxidase, esterase, and up to 11 acid hydrolases. The results obtained indicate that the hydrolases are associated, at least largely, with cytoplasmic particles of lysosomal nature, and suggest further that these particles belong to two, and possibly three, distinct populations, perhaps reflecting the cellular heterogeneity of the tissues. The populations are identified as: (a) the L(19) population, the most important group, containing all 12 hydrolases and characterized by a modal density of about 1.19 in a sucrose-0.2 M KCl gradient; (b) the L(15) population with a modal density of 1.15, a group of apparently incomplete lysosomes containing cathepsin D and a few other enzymes, but very poor in, or entirely devoid of, several acid hydrolases, including cathepsins B and C; (c) the L(30) population, comprising all 12 enzymes and banding together with the nuclei at a density of 1.30 or higher. Lack of success in separating the latter group from the nuclei renders its significance unclear.
W E Bowers; C de Duve
Related Documents :
11048916 - A qtl on pig chromosome 4 affects fatty acid metabolism: evidence from an iberian by la...
2783146 - Effect of 36-hour starvation on in vivo amino acid and glucose uptake by rat brown adip...
8729106 - In vivo 13c nuclear magnetic resonance: applications and current limitations for noninv...
8408646 - Exogenous adenosine triphosphate (atp) preserves proximal tubule microfilament structur...
1100656 - Suitability of peracetic acid for sterilization of media for mycoplasma cultures.
6088546 - Substrate specificities of active transport systems for amino acids in vacuolar-membran...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  32     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1967 Feb 
Date Detail:
Created Date:  2000-09-14     Completed Date:  2000-09-14     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  339-48     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acid Phosphatase / analysis*,  isolation & purification
Hydrolases / analysis*,  isolation & purification
Lymphoid Tissue / enzymology*
Lysosomes / enzymology*
Spleen / enzymology
Subcellular Fractions / enzymology
Reg. No./Substance:
EC 3.-/Hydrolases; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
Download PDF
Copyright © 1967 by The Rockefeller University Press
Received Day: 19 Month: 7 Year: 1966
Print publication date: Day: 1 Month: 2 Year: 1967
Volume: 32 Issue: 2
First Page: 339 Last Page: 348
ID: 2107257
PubMed Id: 10976226

LYSOSOMES IN LYMPHOID TISSUE : II. Intracellular Distribution of Acid Hydrolases
William E. Bowers
Christian de Duve
From The Rockefeller University, New York

Article Categories:
  • Article

Previous Document:  Lysosomes in lymphoid tissue. I. The measurement of hydrolytic activities in whole homogenates.
Next Document:  Lysosomes in lymphoid tissue. III. Influence of various treatments of the animals on the distributio...