Document Detail


Lysosomes in apoptosis.
MedLine Citation:
PMID:  18662570     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysosomes are specialized organelles for protein recycling and as such are involved in the terminal steps of autophagy. However, it has become evident that lysosomes also play an important role in the progression of apoptosis. This latter function seems to be dependent on lysosomal proteases, which need to be released into the cytosol for apoptosis to be efficient. Among the lysosomal proteases, the most abundant are the cysteine cathepsins and the aspartic protease cathepsin D, which seem to be the major apoptosis mediators. This chapter reviews the methods used to study lysosomes and lysosomal proteases.
Authors:
Saska Ivanova; Urska Repnik; Lea Bojic; Ana Petelin; Vito Turk; Boris Turk
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in enzymology     Volume:  442     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-07-29     Completed Date:  2009-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  183-99     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Molecular and Structural Biology, J. Stefan Institute, Ljubljana, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Biological Transport
Cathepsin D / metabolism
Cathepsins / metabolism
Humans
Lysosomes / enzymology*,  metabolism*
Permeability
Substrate Specificity
Chemical
Reg. No./Substance:
EC 3.4.-/Cathepsins; EC 3.4.23.5/Cathepsin D

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