Document Detail

Lysosome-targeted octadecyl-rhodamine B-liposomes enhance lysosomal accumulation of glucocerebrosidase in Gaucher's cells in vitro.
MedLine Citation:
PMID:  23199221     Owner:  NLM     Status:  MEDLINE    
AIM: We hypothesized that liposomes modified with lysosomotropic octadecyl-rhodamine B (Rh) and loaded with therapeutic glucocerebroside velaglucerase alfa (VPRIV™) will improve lysosomal delivery of the enzyme into Gaucher's cells.
MATERIALS & METHODS: Confocal microscopy and flow cytometry were used to evaluate the ability of Rh-modified liposomes loaded with VPRIV to improve the lysosomal targeting in monocyte-derived macrophages and Gaucher's fibroblasts.
RESULTS: Confocal microscopy demonstrated that Rh-modified liposomes localized primarily in the lysosomes. As confirmed by flow cytometry using specific substrate 5-(pentafluorobenzoylamino)fluorescein diglucoside, intralysosomal accumulation of VPRIV in the cells treated with Rh-modified liposomes was significantly increased (up to 68%) relative to the cells treated with plain liposomes or free VPRIV.
CONCLUSION: Rh-modified lysosomotropic liposomes can improve lysosomal accumulation of liposomal enzymes both in nonphagocytic Gaucher's fibroblasts and phagocytic monocyte-derived macrophages.
Ritesh Thekkedath; Alexander Koshkaryev; Vladimir P Torchilin
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-12-02
Journal Detail:
Title:  Nanomedicine (London, England)     Volume:  8     ISSN:  1748-6963     ISO Abbreviation:  Nanomedicine (Lond)     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-10     Completed Date:  2014-02-07     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  101278111     Medline TA:  Nanomedicine (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1055-65     Citation Subset:  IM    
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MeSH Terms
Cell Line
Fibroblasts / metabolism*
Flow Cytometry
Gaucher Disease / enzymology,  metabolism
Glucosylceramidase / metabolism*
Liposomes / chemistry*,  metabolism*
Lysosomes / chemistry*
Microscopy, Confocal
Rhodamines / chemistry*
Grant Support
R01 CA128486/CA/NCI NIH HHS; R01 CA128486/CA/NCI NIH HHS
Reg. No./Substance:
0/Liposomes; 0/Rhodamines; 0/octadecylrhodamine B; EC

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