Document Detail


Lysosome biogenesis mediated by vps-18 affects apoptotic cell degradation in Caenorhabditis elegans.
MedLine Citation:
PMID:  18923146     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Appropriate clearance of apoptotic cells (cell corpses) is an important step of programmed cell death. Although genetic and biochemical studies have identified several genes that regulate the engulfment of cell corpses, how these are degraded after being internalized in engulfing cell remains elusive. Here, we show that VPS-18, the Caenorhabditis elegans homologue of yeast Vps18p, is critical to cell corpse degradation. VPS-18 is expressed and functions in engulfing cells. Deletion of vps-18 leads to significant accumulation of cell corpses that are not degraded properly. Furthermore, vps-18 mutation causes strong defects in the biogenesis of endosomes and lysosomes, thus affecting endosomal/lysosomal protein degradation. Importantly, we demonstrate that phagosomes containing internalized cell corpses are unable to fuse with lysosomes in vps-18 mutants. Our findings thus provide direct evidence for the important role of endosomal/lysosomal degradation in proper clearance of apoptotic cells during programmed cell death.
Authors:
Hui Xiao; Didi Chen; Zhou Fang; Jing Xu; Xiaojuan Sun; Song Song; Jiajia Liu; Chonglin Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-15
Journal Detail:
Title:  Molecular biology of the cell     Volume:  20     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-01-01     Completed Date:  2009-03-27     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21-32     Citation Subset:  IM    
Affiliation:
Key laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Apoptosis / physiology*
Caenorhabditis elegans / cytology,  physiology*
Caenorhabditis elegans Proteins / genetics,  metabolism*
Endocytosis / physiology
Endosomes / metabolism
Humans
Lysosomes / metabolism*
Molecular Sequence Data
Mutation
Phagosomes / metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Sequence Alignment
Vesicular Transport Proteins / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/Recombinant Fusion Proteins; 0/Vesicular Transport Proteins; 0/Vps-18 protein, C elegans
Comments/Corrections

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