| Lysosome biogenesis mediated by vps-18 affects apoptotic cell degradation in Caenorhabditis elegans. | |
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MedLine Citation:
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PMID: 18923146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Appropriate clearance of apoptotic cells (cell corpses) is an important step of programmed cell death. Although genetic and biochemical studies have identified several genes that regulate the engulfment of cell corpses, how these are degraded after being internalized in engulfing cell remains elusive. Here, we show that VPS-18, the Caenorhabditis elegans homologue of yeast Vps18p, is critical to cell corpse degradation. VPS-18 is expressed and functions in engulfing cells. Deletion of vps-18 leads to significant accumulation of cell corpses that are not degraded properly. Furthermore, vps-18 mutation causes strong defects in the biogenesis of endosomes and lysosomes, thus affecting endosomal/lysosomal protein degradation. Importantly, we demonstrate that phagosomes containing internalized cell corpses are unable to fuse with lysosomes in vps-18 mutants. Our findings thus provide direct evidence for the important role of endosomal/lysosomal degradation in proper clearance of apoptotic cells during programmed cell death. |
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Authors:
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Hui Xiao; Didi Chen; Zhou Fang; Jing Xu; Xiaojuan Sun; Song Song; Jiajia Liu; Chonglin Yang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-15 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 20 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-01 Completed Date: 2009-03-27 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 21-32 Citation Subset: IM |
Affiliation:
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Key laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Apoptosis / physiology* Caenorhabditis elegans / cytology, physiology* Caenorhabditis elegans Proteins / genetics, metabolism* Endocytosis / physiology Endosomes / metabolism Humans Lysosomes / metabolism* Molecular Sequence Data Mutation Phagosomes / metabolism Recombinant Fusion Proteins / genetics, metabolism Sequence Alignment Vesicular Transport Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Caenorhabditis elegans Proteins; 0/Recombinant Fusion Proteins; 0/Vesicular Transport Proteins; 0/Vps-18 protein, C elegans |
| Comments/Corrections | |
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