| Lysosomal physiology in Tetrahymena. 3. Pharmacological studies on acid hydrolase release and the ingestion and egestion of dimethylbenzanthracene particles. | |
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MedLine Citation:
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PMID: 4152946 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ingestion of (14)C-labeled 9,10-dimethyl-1,2-benzanthracene particles, the extracellular release of acid phosphatase, ribonuclease, and alpha-glucosidase, and the egestion of preingested dimethylbenzanthracene particles by Tetrahymena taken from logarithmically growing cultures and resuspended in a dilute salt solution were followed in the presence of several pharmacologic agents. Serotonin, caffeine, and, to a lesser extent, dibutyryl cyclic AMP increased the rate of particle ingestion, but did not alter the rate of release of the three acid hydrolases studied. Added catecholamines did not affect either particle ingestion or acid hydrolase release, but particle ingestion was inhibited by the catecholamine antagonists, dichloroisoproterenol, desmethylimipramine, reserpine, and phenoxybenzamine. These drugs also increased the release of acid phosphatase and ribonuclease in 5-h incubations. Desmethylimipramine acted within 1 h to increase acid hydrolase release, but the effect of dichloroisoproterenol developed more slowly and was secondary to a change in cellular content of the hydrolases. Desmethylimipramine increased the energy of activation for the release of acid phosphatase, while dichloroisoproterenol did not. Both of these drugs enhanced the egestion of preingested dimethylbenzanthracene particles, supporting the view that acid hydrolase release occurs through a cytoproct egestion mechanism. Particle ingestion was also inhibited by colchicine, vinblastine, and cytochalasin B, but these agents had no effect on acid hydrolase release, thus further differentiating the properties of the ingestion mechanism from those of the egestion mechanism. It appears that both microtubules and microfilaments play a role in the ingestion process and that this process may be controlled in part by a cyclic AMP-mediated serotoninergic and adrenergic system. |
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Authors:
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T L Rothstein; J J Blum |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 62 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1974 Sep |
Date Detail:
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Created Date: 1974-10-31 Completed Date: 1974-10-31 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 844-59 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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secretion Benz(a)Anthracenes / metabolism* Bucladesine / pharmacology Caffeine / pharmacology Carbon Radioisotopes Colchicine / pharmacology Cytochalasin B / pharmacology Desipramine / pharmacology Endocytosis / drug effects Epinephrine / pharmacology Exocytosis / drug effects Glucosidases / secretion Hydrolases / secretion* Isoproterenol / pharmacology Lysosomes / enzymology* Norepinephrine / pharmacology Phenoxybenzamine / pharmacology Reserpine / pharmacology Ribonucleases / secretion Serotonin / pharmacology Tetrahymena / physiology* Vinblastine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Benz(a)Anthracenes; 0/Carbon Radioisotopes; 14930-96-2/Cytochalasin B; 362-74-3/Bucladesine; 50-47-5/Desipramine; 50-55-5/Reserpine; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 58-08-2/Caffeine; 59-96-1/Phenoxybenzamine; 64-86-8/Colchicine; 7683-59-2/Isoproterenol; 865-21-4/Vinblastine; EC 3.-/Hydrolases; EC 3.1.-/Ribonucleases; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.-/Glucosidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1974 by The Rockefeller University Press Received Day: 8 Month: 11 Year: 1973 Revision Received Day: 23 Month: 4 Year: 1974 Print publication date: Day: 1 Month: 9 Year: 1974 Volume: 62 Issue: 3 First Page: 844 Last Page: 859 ID: 2109214 PubMed Id: 4152946 |
| LYSOSOMAL PHYSIOLOGY IN TETRAHYMENA : III. Pharmacological Studies on Acid Hydrolase Release and the Ingestion and Egestion of Dimethylbenzanthracene Particles | |
| Thomas L. Rothstein | |
| J. J. Blum | |
| From the Department of Physiology and Pharmacology, Duke University Medical Center, Durham, North Carolina 27710 |
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