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Lysosomal physiology in Tetrahymena. 3. Pharmacological studies on acid hydrolase release and the ingestion and egestion of dimethylbenzanthracene particles.
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MedLine Citation:
PMID:  4152946     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ingestion of (14)C-labeled 9,10-dimethyl-1,2-benzanthracene particles, the extracellular release of acid phosphatase, ribonuclease, and alpha-glucosidase, and the egestion of preingested dimethylbenzanthracene particles by Tetrahymena taken from logarithmically growing cultures and resuspended in a dilute salt solution were followed in the presence of several pharmacologic agents. Serotonin, caffeine, and, to a lesser extent, dibutyryl cyclic AMP increased the rate of particle ingestion, but did not alter the rate of release of the three acid hydrolases studied. Added catecholamines did not affect either particle ingestion or acid hydrolase release, but particle ingestion was inhibited by the catecholamine antagonists, dichloroisoproterenol, desmethylimipramine, reserpine, and phenoxybenzamine. These drugs also increased the release of acid phosphatase and ribonuclease in 5-h incubations. Desmethylimipramine acted within 1 h to increase acid hydrolase release, but the effect of dichloroisoproterenol developed more slowly and was secondary to a change in cellular content of the hydrolases. Desmethylimipramine increased the energy of activation for the release of acid phosphatase, while dichloroisoproterenol did not. Both of these drugs enhanced the egestion of preingested dimethylbenzanthracene particles, supporting the view that acid hydrolase release occurs through a cytoproct egestion mechanism. Particle ingestion was also inhibited by colchicine, vinblastine, and cytochalasin B, but these agents had no effect on acid hydrolase release, thus further differentiating the properties of the ingestion mechanism from those of the egestion mechanism. It appears that both microtubules and microfilaments play a role in the ingestion process and that this process may be controlled in part by a cyclic AMP-mediated serotoninergic and adrenergic system.
Authors:
T L Rothstein; J J Blum
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  62     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1974 Sep 
Date Detail:
Created Date:  1974-10-31     Completed Date:  1974-10-31     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  844-59     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / secretion
Benz(a)Anthracenes / metabolism*
Bucladesine / pharmacology
Caffeine / pharmacology
Carbon Radioisotopes
Colchicine / pharmacology
Cytochalasin B / pharmacology
Desipramine / pharmacology
Endocytosis / drug effects
Epinephrine / pharmacology
Exocytosis / drug effects
Glucosidases / secretion
Hydrolases / secretion*
Isoproterenol / pharmacology
Lysosomes / enzymology*
Norepinephrine / pharmacology
Phenoxybenzamine / pharmacology
Reserpine / pharmacology
Ribonucleases / secretion
Serotonin / pharmacology
Tetrahymena / physiology*
Vinblastine / pharmacology
Chemical
Reg. No./Substance:
0/Benz(a)Anthracenes; 0/Carbon Radioisotopes; 14930-96-2/Cytochalasin B; 362-74-3/Bucladesine; 50-47-5/Desipramine; 50-55-5/Reserpine; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; 58-08-2/Caffeine; 59-96-1/Phenoxybenzamine; 64-86-8/Colchicine; 7683-59-2/Isoproterenol; 865-21-4/Vinblastine; EC 3.-/Hydrolases; EC 3.1.-/Ribonucleases; EC 3.1.3.2/Acid Phosphatase; EC 3.2.1.-/Glucosidases
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Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1974 by The Rockefeller University Press
Received Day: 8 Month: 11 Year: 1973
Revision Received Day: 23 Month: 4 Year: 1974
Print publication date: Day: 1 Month: 9 Year: 1974
Volume: 62 Issue: 3
First Page: 844 Last Page: 859
ID: 2109214
PubMed Id: 4152946

LYSOSOMAL PHYSIOLOGY IN TETRAHYMENA : III. Pharmacological Studies on Acid Hydrolase Release and the Ingestion and Egestion of Dimethylbenzanthracene Particles
Thomas L. Rothstein
J. J. Blum
From the Department of Physiology and Pharmacology, Duke University Medical Center, Durham, North Carolina 27710


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