| Lysosomal membrane permeabilization is involved in curcumin-induced apoptosis of A549 lung carcinoma cells. | |
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MedLine Citation:
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PMID: 21874542 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We previously reported that curcumin inhibited lung cancer A549 cells growth and promoted cell apoptosis in vitro. In this study, we further examined the apoptosis-related parameters, including lysosomal damage and cathepsin activation, in A549 cells exposed to curcumin. We found that curcumin caused lysosomal membrane permeabilization (LMP) and cytosolic relocation of cathepsin B (cath B) and cathepsin D (cath D). However, only Z-FA-fmk (a cath B inhibitor) but not pepstatin A (a cath D inhibitor) inhibited curcumin-induced cell apoptosis, mitochondrial membrane potential loss, and cytochrome c release. The antioxidant N-acetylcysteine and glutathione attenuated LMP, suggesting that lysosomal destabilization was dependent on the elevation of reactive oxygen species and which precedes mitochondrial dysfunction. These findings indicated a novel pathway for curcumin regulation of ROS-lysosomal-mitochondrial pathway and provided the key mechanism of regulation of LMP in cell apoptosis, which may be exploited for cancer treatment. |
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Authors:
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Qing-Yong Chen; Jian-Guo Shi; Qing-Hua Yao; De-Min Jiao; Yan-Yi Wang; Hui-Zhen Hu; Yu-Quan Wu; Jia Song; Jie Yan; Li-Jun Wu |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-28 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: - ISSN: 1573-4919 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-8-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Respiratory Disease, The 117th Hospital of PLA, Hang Zhou, 310013, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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