Document Detail

Lysosomal enzyme activities in parenchymal and nonparenchymal liver cells isolated from young, adult and old rats.
MedLine Citation:
PMID:  994558     Owner:  NLM     Status:  MEDLINE    
Parenchymal and nonparenchymal cells were isolated from the livers of female BN/BiRij rats, aged 3, 12, 24 and 30-35 months, by means of enzymatic techniques. About 70% of the cells in the nonparenchymal cell suspensions were endothelial cells and 25% were Kupffer cells. More than 90% of the isolated parenchymal, Kupffer and endothelial cells were viable as judged by trypan blue exclusion and ultrastructural appearance. The age-related changes in the specific activities of the lysosomal enzymes acid phosphatase, beta-galactosidase, cathepsin D and arylsulphatase B in parenchymal and nonparenchymal cells showed no correlated behavior. The most prominent change was observed for the cathepsin D activity in parenchymal cells, which nearly triples during the lifespan of the rat. A comparison of the activities obtained with homogenates of the whole liver and with parenchymal and nonparenchymal cells revealed that aging changes in lysosomal enzyme activities in homogenates should be carefully interpreted, since opposite patterns of change were often observed in the activities in parenchymal cells and in nonparenchymal cells.
D L Knook; E C Sleyster
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  5     ISSN:  0047-6374     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  1976  
Date Detail:
Created Date:  1977-01-28     Completed Date:  1977-01-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  389-98     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / metabolism
Age Factors
Arylsulfatases / metabolism
Cathepsins / metabolism
Cell Survival
Galactosidases / metabolism
Kupffer Cells / enzymology
Liver / cytology,  enzymology*
Lysosomes / enzymology*
Rats, Inbred BN
Reg. No./Substance:
EC Phosphatase; EC; EC 3.2.1.-/Galactosidases; EC 3.4.-/Cathepsins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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