| Lysosomal accumulation of mTOR is enhanced by rapamycin. | |
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MedLine Citation:
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PMID: 21063721 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The mammalian target of rapamycin (mTOR) is a key regulator of cell growth that integrates signals from growth factors and nutrients. Recent studies have shown that an mTOR-containing complex, mTORC1, is targeted to lysosomes in the presence of amino acids and activated by Rheb GTPase resident in that compartment. In this study, we found that treatment with the mTOR inhibitors rapamycin and Torin1 significantly enhanced lysosomal accumulation of mTOR and Raptor. This phenomenon was not observed in the absence of amino acids but was restored upon addition of L: -leucine or protein synthesis inhibitors. mTOR was not concentrated in autophagosomes that were induced by rapamycin. These results suggest that the lysosome harbors both active and inactive forms of mTOR in the presence of amino acids. |
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Authors:
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Yuki Ohsaki; Michitaka Suzuki; Yuki Shinohara; Toyoshi Fujimoto |
Publication Detail:
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Type: Journal Article Date: 2010-11-10 |
Journal Detail:
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Title: Histochemistry and cell biology Volume: 134 ISSN: 1432-119X ISO Abbreviation: Histochem. Cell Biol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9506663 Medline TA: Histochem Cell Biol Country: Germany |
Other Details:
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Languages: eng Pagination: 537-44 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, 466-8550, Japan, yohsaki@med.nagoya-u.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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