| Lysophospholipase and the metabolism of lysophosphatidylcholine in isolated bovine rod outer segments. | |
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MedLine Citation:
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PMID: 2920784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of bovine rod outer segments (ROS) with radiolabeled palmitic acid (16:0) and lysophosphatidylcholines (lysoPC) radiolabeled in either the fatty acid or the choline group indicated the presence of a lysophospholipase activity that is unaffected by Ca2+. In the presence of ATP, Mg2+ and CoA and acyl CoA:lysophospholipid acytransferase activity is evident, and free fatty acids, including those released by lysophospholipase activity, are esterified to membrane phospholipids. At low concentrations of lysoPC, 68% of it is acylated to form phosphatidylcholine (PC) and 24% is converted to glycerophosphocholine (GPC) and fatty acid per hour. As the concentration of lysoPC increases lysophospholipase activity increases, acyl-CoA:lysophospholipid acyltransferase activity decreases, and the proportion of lysoPC converted to PC decreases. The rate of production of lysophospholipids in vitro under phospholipase A-stimulatory conditions exceeds the rate at which it can be removed by 5-10-fold. This suggests the possibility that an early step in light, anoxia- or hypoxia-induced damage to photoreceptor cells may be activation of the phospholipase A endogenous to ROS. |
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Authors:
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W F Zimmerman; S Keys |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental eye research Volume: 48 ISSN: 0014-4835 ISO Abbreviation: Exp. Eye Res. Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-04-14 Completed Date: 1989-04-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370707 Medline TA: Exp Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 69-76 Citation Subset: IM |
Affiliation:
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Department of Biology, Amherst College, MA 01002. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Acylglycerophosphocholine O-Acyltransferase
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metabolism Adenosine Triphosphate / metabolism Animals Cattle Coenzyme A / metabolism Fatty Acids / metabolism Glycerylphosphorylcholine / metabolism Lysophosphatidylcholines / metabolism* Lysophospholipase / metabolism* Magnesium / metabolism Palmitic Acids / metabolism Phosphatidylcholines / biosynthesis Phospholipases / metabolism* Phosphorylcholine / metabolism Photoreceptor Cells / enzymology* Rod Cell Outer Segment / enzymology* Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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EY00675/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Lysophosphatidylcholines; 0/Palmitic Acids; 0/Phosphatidylcholines; 107-73-3/Phosphorylcholine; 56-65-5/Adenosine Triphosphate; 563-24-6/Glycerylphosphorylcholine; 7439-95-4/Magnesium; 85-61-0/Coenzyme A; EC 2.3.1.23/1-Acylglycerophosphocholine O-Acyltransferase; EC 3.1.-/Phospholipases; EC 3.1.1.5/Lysophospholipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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