| Lysophosphatidylcholine increases the secretion of matrix metalloproteinase 2 through the activation of NADH/NADPH oxidase in cultured aortic endothelial cells. | |
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MedLine Citation:
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PMID: 11223425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix metalloproteinases (MMPs) play a pivotal role in angiogenesis, atherogenesis, vascular remodeling after vascular injury, and instability of atherosclerotic plaque. The present study was undertaken to investigate the effect of lysophosphatidylcholine, a major component of oxidized low density lipoprotein (LDL), on the regulation of MMPs in cultured bovine aortic endothelial cells (BAECs). Furthermore, we explored the potential role of oxidative stress in the regulation of MMP. LPC increased the secretion of gelatinolytic activity, as well as, protein of MMP-2 from BAECs. The stimulation of BAEC with superoxide increased the production of MMP-2 and it also induced its activation. Electron spin resonance (ESR) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as spin trap agent demonstrated that lysophosphatidycholine (LPC) induced generation of reactive oxygen (ROS) species from BAECs. The inhibition of NADH/NADPH oxidase, one of the potential sources of superoxide in endothelial cells, attenuated the effect of LPC. Our findings suggest that LPC might activate the endothelial NADH/NADPH oxidase to enhance superoxide production, and it might, in turn, enhance MMP-2 induction. |
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Authors:
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N Inoue; S Takeshita; D Gao; T Ishida; S Kawashima; H Akita; R Tawa; H Sakurai; M Yokoyama |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Atherosclerosis Volume: 155 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 2001 Mar |
Date Detail:
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Created Date: 2001-03-06 Completed Date: 2001-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: Ireland |
Other Details:
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Languages: eng Pagination: 45-52 Citation Subset: IM |
Affiliation:
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First Department of Internal Medicine, School of Medicine, Kobe University, 7-5-2 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta Blotting, Western Cattle Cells, Cultured Electron Spin Resonance Spectroscopy Endothelium, Vascular / enzymology*, metabolism Enzyme Activation Lysophosphatidylcholines / pharmacology* Matrix Metalloproteinase 2 / secretion* NAD / metabolism* NADP / metabolism* NADPH Oxidase / metabolism* Oxidative Stress Precipitin Tests Reactive Oxygen Species / metabolism Superoxides / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Lysophosphatidylcholines; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 53-59-8/NADP; 53-84-9/NAD; EC 1.6.3.1/NADPH Oxidase; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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