Document Detail


Lysophosphatidic acid induces process retraction in CG-4 line oligodendrocytes and oligodendrocyte precursor cells but not in differentiated oligodendrocytes.
MedLine Citation:
PMID:  14622125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lysophosphatidic acid is a growth factor-like signalling phospholipid. We demonstrate here that lysophosphatidic acid induces process retraction in central glia-4 cells and oligodendrocyte precursors. This lysophosphatidic acid effect is rapid and concentration-dependent and results in cell rounding. It is inhibited by pre-treatment of cells with C3 exoenzyme, a specific inhibitor of Rho, or with Y-27632, a specific inhibitor of ROCK, a downstream kinase of Rho. Processes of differentiated central glia-4 oligodendrocytes were insensitive to lysophosphatidic acid treatment but cell bodies became phase dark, indicating cell spreading on the poly-l-lysine substratum. RT-PCR and Western blot analyses indicate that oligodendrocyte precursors and mature oligodendrocytes express mRNA and protein for LPA1, one of several LPA receptors. Thus lysophosphatidic acid may be signalling to Rho and stimulating actomyosin contraction in precursor oligodendrocytes by this family of receptors. The results show that lysophosphatidic acid signalling pathways influence retraction of processes in oligodendrocyte precursors but that this effect changes as oligodendrocytes differentiate.
Authors:
John Dawson; Neil Hotchin; Sian Lax; Martin Rumsby
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  87     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-19     Completed Date:  2004-01-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  947-57     Citation Subset:  IM    
Affiliation:
Department of Biology, University of York, York, UK. jcd110@york.ac.uk
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MeSH Terms
Descriptor/Qualifier:
ADP Ribose Transferases / pharmacology
Amides / pharmacology
Animals
Botulinum Toxins / pharmacology
Cell Differentiation / physiology
Cell Line
Cell Surface Extensions / drug effects*
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Lysophospholipids / pharmacology*
Oligodendroglia / cytology,  drug effects*,  metabolism
Pyridines / pharmacology
RNA, Messenger / biosynthesis
Rats
Receptors, G-Protein-Coupled / biosynthesis,  genetics
Receptors, Lysophosphatidic Acid
Signal Transduction / drug effects,  physiology
Stem Cells / cytology,  drug effects*,  metabolism
Chemical
Reg. No./Substance:
0/Amides; 0/Botulinum Toxins; 0/Enzyme Inhibitors; 0/Lysophospholipids; 0/Pyridines; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 0/Receptors, Lysophosphatidic Acid; 138381-45-0/Y 27632; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.-/exoenzyme C3, Clostridium botulinum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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