| Lysophosphatidic acid induces process retraction in CG-4 line oligodendrocytes and oligodendrocyte precursor cells but not in differentiated oligodendrocytes. | |
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MedLine Citation:
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PMID: 14622125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lysophosphatidic acid is a growth factor-like signalling phospholipid. We demonstrate here that lysophosphatidic acid induces process retraction in central glia-4 cells and oligodendrocyte precursors. This lysophosphatidic acid effect is rapid and concentration-dependent and results in cell rounding. It is inhibited by pre-treatment of cells with C3 exoenzyme, a specific inhibitor of Rho, or with Y-27632, a specific inhibitor of ROCK, a downstream kinase of Rho. Processes of differentiated central glia-4 oligodendrocytes were insensitive to lysophosphatidic acid treatment but cell bodies became phase dark, indicating cell spreading on the poly-l-lysine substratum. RT-PCR and Western blot analyses indicate that oligodendrocyte precursors and mature oligodendrocytes express mRNA and protein for LPA1, one of several LPA receptors. Thus lysophosphatidic acid may be signalling to Rho and stimulating actomyosin contraction in precursor oligodendrocytes by this family of receptors. The results show that lysophosphatidic acid signalling pathways influence retraction of processes in oligodendrocyte precursors but that this effect changes as oligodendrocytes differentiate. |
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Authors:
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John Dawson; Neil Hotchin; Sian Lax; Martin Rumsby |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 87 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-11-19 Completed Date: 2004-01-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 947-57 Citation Subset: IM |
Affiliation:
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Department of Biology, University of York, York, UK. jcd110@york.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ADP Ribose Transferases
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pharmacology Amides / pharmacology Animals Botulinum Toxins / pharmacology Cell Differentiation / physiology Cell Line Cell Surface Extensions / drug effects* Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology Lysophospholipids / pharmacology* Oligodendroglia / cytology, drug effects*, metabolism Pyridines / pharmacology RNA, Messenger / biosynthesis Rats Receptors, G-Protein-Coupled / biosynthesis, genetics Receptors, Lysophosphatidic Acid Signal Transduction / drug effects, physiology Stem Cells / cytology, drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amides; 0/Botulinum Toxins; 0/Enzyme Inhibitors; 0/Lysophospholipids; 0/Pyridines; 0/RNA, Messenger; 0/Receptors, G-Protein-Coupled; 0/Receptors, Lysophosphatidic Acid; 138381-45-0/Y 27632; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.-/exoenzyme C3, Clostridium botulinum |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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