| Lysis of tumor cells by antibody and complement. VII. Complement-dependent 86Rb release--a nonlethal event? | |
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MedLine Citation:
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PMID: 185297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The guinea pig hepatoma (line-1) treated with anti-Forssman antibody (TA) and GPC sequentially released 86Rb, 14C from 14C aminoidobutyric acid and failed to exclude trypan blue. Incubation of TA with fluid phase GPC for 1 min caused maximal 86Rb release; however, if the GPC was removed at this time, the cells were not subsequently killed. Using a number of naturally occurring human sera deficient in a complement component we have shown 86Rb release requires the binding of the complement components 1 through 8, but there was no absolute requirement for C9. Irreversible damage to the cell as measured by 14C AIB release or uptake of trypan blue required the complete sequence of complete sequence of complement components. These observations indicate that 86Rb release is not a relible indicator cytotoxicity. |
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Authors:
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M D Boyle; S H Ohanian; T Borsos |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 117 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1976 Oct |
Date Detail:
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Created Date: 1976-12-30 Completed Date: 1976-12-30 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1346-50 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies* Binding Sites Carcinoma, Hepatocellular / immunology* Complement C8 / metabolism Complement C9 / metabolism Complement System Proteins / metabolism* Cytotoxicity Tests, Immunologic Guinea Pigs Humans Liver Neoplasms / immunology* Rubidium* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Complement C8; 0/Complement C9; 7440-17-7/Rubidium; 9007-36-7/Complement System Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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