Document Detail

Lysis and dissolution of cells and envelopes of an extremely halophilic bacterium.
MedLine Citation:
PMID:  5874536     Owner:  NLM     Status:  MEDLINE    
Kushner, D. J. (National Research Council, Ottawa, Ontario, Canada). Lysis and dissolution of cells and envelopes of an extremely halophilic bacterium. J. Bacteriol. 87:1147-1156. 1964.-Envelopes of the extremely halophilic bacterium, Halobacterium cutirubrum, disintegrate in the absence of salt to form much smaller particles. Extensive proteolytic breakdown to compounds of low molecular weight is not involved in this process or in the lysis of cells in the absence of salt. NaCl is much more effective than KCl or NH(4)Cl in preserving the integrity of intact cells, but is only slightly more effective in preserving the integrity of mechanically prepared envelopes, of cells made permeable by treatment with acid, and of cells made permeable by formalin fixation followed by exposure to water. MgCl(2) is much more effective in preserving the integrity of these preparations than of intact cells. The results suggest that the exterior cell surface has sites specifically requiring Na(+) to maintain their integrity, whereas the interior surface has sites whose integrity is maintained at least as well by K(+) or NH(4) (+) as by Na(+).
D J Kushner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  87     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1964 May 
Date Detail:
Created Date:  1967-04-11     Completed Date:  1967-04-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1147-56     Citation Subset:  IM    
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MeSH Terms
Ammonium Chloride / pharmacology
Cell Membrane / drug effects
Cell Wall / analysis,  drug effects
Halobacterium / drug effects*
Magnesium / pharmacology
Potassium Chloride / pharmacology
Sodium Chloride / pharmacology*
Reg. No./Substance:
12125-02-9/Ammonium Chloride; 7439-95-4/Magnesium; 7447-40-7/Potassium Chloride; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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