Document Detail


Lysine decarboxylase activity in broth and agar media.
MedLine Citation:
PMID:  5002895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four lysine decarboxylase media were studied by testing them with 305 Enterobacteriaceae and 42 nonfermenting bacilli. A comparison was made between lysine decarboxylase broth medium (Moeller base) and Johnson's semisolid agar without lactose and Bachrach's broth medium and lysine-agar slants which contain lactose. The nonlactose media, lysine decarboxylase broth and the semisolid medium of Johnson, were the best media for use with all of the bacteria studied. The exclusion of lactose from lysine decarboxylase medium seems desirable to extend the usefulness of this medium among members of the Enterobacteriaceae. When the results with lysine decarboxylase broth and Johnson's semisolid medium without lactose were compared, a 6% difference existed between the results obtained with lysine decarboxylase broth and Johnson's semisolid agar. When the results with Bachrach's broth and lysine-agar slants with lactose were compared, a 1% difference existed between Bachrach's broth and the agar slant method. At times, reading and interpretation were difficult because of intermediate degrees of color change. The inability of Pseudomonas aeruginosa or Herellea to utilize glucose under the anaerobic condition of the medium makes the lysine decarboxylase test an undesirable procedure for these organisms. Of the four test media used, the lysine-lactose-agar slants seemed to be the least desirable because of the more frequent occurrence of indistinct color reactions and shifts in color.
Authors:
H R Elston
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  22     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1971 Dec 
Date Detail:
Created Date:  1972-04-04     Completed Date:  1972-04-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1091-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agar*
Bacteria / enzymology
Bacteriological Techniques / standards*
Carboxy-Lyases / metabolism*
Color
Culture Media*
Enterobacteriaceae / enzymology,  isolation & purification*
Indicators and Reagents
Lactose
Lysine
Pseudomonas aeruginosa / enzymology,  isolation & purification
Species Specificity
Chemical
Reg. No./Substance:
0/Culture Media; 0/Indicators and Reagents; 56-87-1/Lysine; 63-42-3/Lactose; 9002-18-0/Agar; EC 4.1.1.-/Carboxy-Lyases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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