Document Detail


Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome.
MedLine Citation:
PMID:  19153599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recruitment of substrates to the 26S proteasome usually requires covalent attachment of the Lys48-linked polyubiquitin chain. In contrast, modifications with the Lys63-linked polyubiquitin chain and/or monomeric ubiquitin are generally thought to function in proteasome-independent cellular processes. Nevertheless, the ubiquitin chain-type specificity for the proteasomal targeting is still poorly understood, especially in vivo. Using mass spectrometry, we found that Rsp5, a ubiquitin-ligase in budding yeast, catalyzes the formation of Lys63-linked ubiquitin chains in vitro. Interestingly, the 26S proteasome degraded well the Lys63-linked ubiquitinated substrate in vitro. To examine whether Lys63-linked ubiquitination serves in degradation in vivo, we investigated the ubiquitination of Mga2-p120, a substrate of Rsp5. The polyubiquitinated p120 contained relatively high levels of Lys63-linkages, and the Lys63-linked chains were sufficient for the proteasome-binding and subsequent p120-processing. In addition, Lys63-linked chains as well as Lys48-linked chains were detected in the 26S proteasome-bound polyubiquitinated proteins. These results raise the possibility that Lys63-linked ubiquitin chain also serves as a targeting signal for the 26S proteaseome in vivo.
Authors:
Yasushi Saeki; Tai Kudo; Takayuki Sone; Yoshiko Kikuchi; Hideyoshi Yokosawa; Akio Toh-e; Keiji Tanaka
Related Documents :
12948749 - Archaeal proteasomes: potential in metabolic engineering.
8684489 - Autocatalytic processing of the 20s proteasome.
11564559 - Extended peptide-based inhibitors efficiently target the proteasome and reveal overlapp...
9792109 - The functional importance of structural differences between the mannitol-specific iiama...
17218279 - Altered specificity of a aaa+ protease.
10698189 - Substrate-induced down-regulation of human type 2 deiodinase (hd2) is mediated through ...
6812049 - Molecular defect in combined beta-galactosidase and neuraminidase deficiency in man.
9089289 - Glutamine-330 is not essential for activity in isopenicillin n synthase from aspergillu...
21418019 - Structural and functional properties of kunitz proteinase inhibitors from leguminosae: ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-15
Journal Detail:
Title:  The EMBO journal     Volume:  28     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-19     Completed Date:  2009-03-26     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  359-71     Citation Subset:  IM    
Affiliation:
Laboratory of Frontier Science, Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cyclin-Dependent Kinase Inhibitor Proteins
Endosomal Sorting Complexes Required for Transport
Lysine / chemistry*
Mass Spectrometry / methods
Membrane Proteins
Models, Biological
Plasmids / metabolism
Polyubiquitin / chemistry*
Proteasome Endopeptidase Complex / metabolism*
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / metabolism*
Signal Transduction
Trans-Activators / metabolism
Transcription Factors
Ubiquitin / chemistry
Ubiquitin-Protein Ligase Complexes / metabolism*
p120 GTPase Activating Protein / metabolism
Chemical
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor Proteins; 0/Endosomal Sorting Complexes Required for Transport; 0/MGA2 protein, S cerevisiae; 0/Membrane Proteins; 0/SIC1 protein, S cerevisiae; 0/SPT23 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 0/Transcription Factors; 0/Ubiquitin; 0/p120 GTPase Activating Protein; 120904-94-1/Polyubiquitin; 56-87-1/Lysine; EC 3.4.25.1/Proteasome Endopeptidase Complex; EC 3.4.99.-/ATP dependent 26S protease; EC 6.3.2.19/RSP5 protein, S cerevisiae; EC 6.3.2.19/Ubiquitin-Protein Ligase Complexes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  TGF-beta-induced epithelial to mesenchymal transition.
Next Document:  Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes.