| Lymphokine release from human lymphomononuclear cells after co-culture with isolated pancreatic islets: effects of islet species, long-term culture, and monocyte-macrophage cell removal. | |
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MedLine Citation:
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PMID: 10857768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study evaluated the release of Th1 and Th2 cytokines from human lymphomononuclear cells (LMC) in response to purified human (HI) or bovine (BI) islets, and the role of long-term (3-4 weeks) islet culture and removal of monocyte-macrophage cells. The results showed that HI and BI caused a similar increase of the release of gamma interferon (IFN), IL-2 and IL-6 from LMC, whereas BI had a more marked effect than HI on IL-10 release. Culturing the islets had possible positive effects (reduction of IFN and IL-2), but also potentially negative effects (increase of TNF). Removal of monocyte-macrophage cells determined a significant reduction of IL-6, IL-10 and TNF production in response to xeno-islets. |
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Authors:
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L Marselli; P Marchetti; C Tellini; R Giannarelli; C Lencioni; S Del Guerra; R Lupi; M Carmellini; F Mosca; R Navalesi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cytokine Volume: 12 ISSN: 1043-4666 ISO Abbreviation: Cytokine Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-10-03 Completed Date: 2000-10-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 503-5 Citation Subset: IM |
Affiliation:
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Dipartimento di Endocrinologia e Metabolismo, University of Pisa, Italy. metabpri@immr.med.unipi.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cells, Cultured Coculture Techniques Cytokines / biosynthesis* Humans Interferons / biosynthesis Interleukin-10 / biosynthesis Interleukin-2 / biosynthesis Interleukin-6 / biosynthesis Islets of Langerhans / cytology, metabolism* Leukocytes, Mononuclear / cytology, metabolism* Macrophages / cytology, metabolism Monocytes / cytology, metabolism Time Factors Tumor Necrosis Factor-alpha / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Interleukin-2; 0/Interleukin-6; 0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; 9008-11-1/Interferons |
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