Document Detail

Lymphocytic infiltration and expression of inducible nitric oxide synthase in human duodenal and colonic mucosa is a characteristic feature of ankylosing spondylitis.
MedLine Citation:
PMID:  14677189     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: In patients with ankylosing spondylitis (AS), inflammatory processes have been detected in the ileal and colonic mucosa. The inducible isoform of nitric oxide synthase (iNOS) may be expressed early in the inflammatory process. We investigated iNOS activity and lymphocytic infiltration in the duodenum and colon in patients with AS and ulcerative colitis compared with controls.
METHODS: Gastroscopy with duodenal biopsies and/or colonoscopy with biopsies were conducted in 42 patients with AS treated or not treated with nonsteroidal antiinflammatory drugs (NSAID), in 15 with ulcerative colitis, and in 46 controls. Lymphocytic infiltration in the lamina propria and intraepithelial infiltration were quantified by histological score. iNOS expression was assessed by immunohistochemistry with monoclonal antibodies, and iNOS activity was determined by radiochemical assay.
RESULTS: Endoscopic examination of the gastroduodenal or colonic mucosa did not reveal macroscopic lesions in the AS patients. In the duodenum, mucosal lymphocytic infiltration was found in 83.3% of the AS group compared to 48.6% of controls (p = 0.02), and was independent of the NSAID intake. Intraepithelial lymphocyte infiltration was increased in both duodenum and colon in AS patients compared to controls. iNOS activity in duodenum and colon and expression of iNOS protein in lamina propria inflammatory cells was increased in AS patients compared to controls.
CONCLUSION: Lymphocytic infiltration and iNOS expression and activity were detected in duodenal and colonic mucosa from patients with AS. Such findings may indicate an inflammatory process in the small intestine and colon of patients with AS.
Dominique Lamarque; Jeanne Tran Van Nhieu; Maxime Breban; Christophe Bernardeau; Nadine Martin-Garcia; Zoltan Szepes; Jean-Charles Delchier; Brendan Whittle; Pascal Claudepierre
Related Documents :
323299 - Antidiarrheal effects of dihydroergotamine.
19598329 - Colonic adenoma patients have higher incidence of hyperplastic polyps on surveillance c...
18810779 - Resection and primary anastomosis with or without modified blow-hole colostomy for sigm...
6580489 - Immunodiagnosis of colorectal cancer: clinical evaluation of tennessee antigen (tenagen).
658769 - Is there a myoelectrical abnormality in the irritable colon syndrome?
2265659 - Detrusor sphincter dyssynergia syndrome. a new syndrome and its treatment by external s...
18086659 - Physical injuries caused by a transient loss of consciousness: main clinical characteri...
1949969 - Ten cases of acute lead intoxication among bridge workers in louisiana.
23741569 - Right upper lobe tracheal bronchus: anesthetic challenge in one-lung ventilated patient...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of rheumatology     Volume:  30     ISSN:  0315-162X     ISO Abbreviation:  J. Rheumatol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-12-16     Completed Date:  2004-01-05     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  7501984     Medline TA:  J Rheumatol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  2428-36     Citation Subset:  IM    
Institut National de la Santé et de la Recherche Médicale (INSERM) U99, Department of Gastroenterology, AP-HP Hôpital Henri Mondor, F-94010 Créteil, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
Case-Control Studies
Cell Movement
Colon / enzymology,  metabolism,  microbiology,  pathology
Duodenum / enzymology,  metabolism,  microbiology,  pathology
Helicobacter pylori / isolation & purification
Interferon-gamma / metabolism
Interleukin-10 / metabolism
Interleukin-2 / metabolism
Intestinal Mucosa / enzymology*,  metabolism,  microbiology,  pathology*
Lymphocytes / pathology*,  physiology
Middle Aged
Nitric Oxide Synthase / metabolism*
Nitric Oxide Synthase Type II
Spondylitis, Ankylosing / enzymology*,  metabolism,  microbiology,  pathology*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Interleukin-2; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma; EC protein, human; EC Oxide Synthase; EC Oxide Synthase Type II
Comment In:
J Rheumatol. 2003 Nov;30(11):2306-7   [PMID:  14677167 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Serum cytokine profiles in patients with adult onset Still's disease.
Next Document:  The effect of control and self-medication of chronic gout in a developing country. Outcome after 10 ...