Document Detail


Lymphocytic choriomeningitis virus mx strain does not induce the expression of tumor-associated carbonic anhydrase IX in persistently infected HeLa cells.
MedLine Citation:
PMID:  16599186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Summary. - Lymphocytic choriomeningitis virus (LCMV) is an arenavirus that readily causes persistent infections, in which it does not interfere with vital functions of the cells, but can affect expression of "luxurious" genes. MX strain of LCMV (MX LCMV) has been identified as an agent transmissible by cell-to-cell contact in the human carcinoma MaTu cells grown in a mixed culture with HeLa cells. When compared to uninfected HeLa, the MaTu-MX-infected HeLa cells, to which the virus was transmitted via co-cultivation with mitomycin C-treated MaTu cells, showed an elevated expression of a protein called MN, suggesting that MN can be induced by MX LCMV. MN protein was later identified as the carbonic anhydrase isoform IX (CA IX), whose expression has been predominantly associated with hypoxic tumors of poor prognosis. Since the proposal that MX LCMV can induce such a cancer-related protein could substantially change our view on the biology of LCMV-host interaction we undertook its verification. Instead of co-cultivation, we used MaTu cell-free extracts to transmit MX LCMV to HeLa cells. These cells were then grown for more than 30 passages, but the level of MN/CA IX did not increase throughout the whole cultivation period as compared to uninfected HeLa cells. Moreover, a treatment of MaTu-MX-infected HeLa cells with ribavirin eliminated the virus, but did not reduce the MN/CA IX expression. Our results clearly showed that MN/CA IX is independent of MX LCMV and that the virus itself does not influence the MN/CA IX level in HeLa cells.
Authors:
M Labudová; J Tomásková; M Kaluzová; J Pastorek; S Pastoreková
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta virologica     Volume:  50     ISSN:  0001-723X     ISO Abbreviation:  Acta Virol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-04-07     Completed Date:  2006-05-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370401     Medline TA:  Acta Virol     Country:  Slovakia    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Affiliation:
Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Díbravská cesta 9, 845 05 Bratislava, Slovak Republic.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / biosynthesis*
Carbonic Anhydrases / biosynthesis*
Enzyme Induction
Hela Cells
Humans
Lymphocytic choriomeningitis virus / drug effects,  physiology*
Ribavirin / pharmacology
Tumor Markers, Biological / biosynthesis*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Tumor Markers, Biological; 36791-04-5/Ribavirin; EC 4.2.1.1/CA9 protein, human; EC 4.2.1.1/Carbonic Anhydrases

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