Document Detail


Lymphocytes proliferate in blood and lymph nodes following interleukin-2 therapy in addition to highly active antiretroviral therapy.
MedLine Citation:
PMID:  11807298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Substantial redistribution of lymphocytes occurs upon the initiation of highly active antiretroviral therapy (HAART) and immune-based HIV therapies. OBJECTIVE: To evaluate the relative contribution of apoptosis and proliferation to changes in lymphocyte populations in peripheral blood and lymph node resulting from interleukin-2 (IL-2) therapy in patients receiving stable HAART. METHODS: Lymphocyte apoptosis was analyzed on various subtypes using fluorescence activated cell sorting with an annexin-V antibody in peripheral blood and by the TUNEL (terminal uridine nucleotide end labelling) method in corresponding lymph node sections. Lymphocyte proliferation was evaluated using an antibody against the cell cycle-associated marker Ki-67 (MIB-1) in peripheral blood and lymph nodes. RESULTS: A transient increase in apoptosis was seen in peripheral blood and lymph nodes during a cycle of subcutaneous IL-2. A pronounced proliferative effect of IL-2 (from 6.4% of total lymphocytes in patients only treated with HAART to 23.4% in those treated with HAART + IL-2) was detected in peripheral blood, affecting the CD4, CD8 and CD16/56 subsets to a similar extent. Remarkably, the proliferative effect also occurred in lymphoid tissues. While the lymph node structure gradually disintegrated over 24 months in some individuals, the amount of proliferating lymphocytes, including CD4 cells, B cells and follicular dendritic cells, greatly increased upon IL-2, while HIV RNA load in lymph nodes remained unaffected. CONCLUSION: These results show that IL-2 leads to lymphocyte proliferation in peripheral blood and lymph nodes without an impact on viral load in lymphoid tissue. These results have important implications for attempts to reconstitute the immune system in HIV disease.
Authors:
Ulrich R Hengge; Carsten Borchard; Stefan Esser; Margit Schröder; Alireza Mirmohammadsadegh; Manfred Goos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS (London, England)     Volume:  16     ISSN:  0269-9370     ISO Abbreviation:  AIDS     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-24     Completed Date:  2002-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8710219     Medline TA:  AIDS     Country:  England    
Other Details:
Languages:  eng     Pagination:  151-60     Citation Subset:  IM; X    
Affiliation:
Department of Dermatology, Venerology and Allergology, University of Essen, Essen, Germany. ulrich.henge@uni-essen.de
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MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / therapeutic use
Antiretroviral Therapy, Highly Active
Apoptosis / drug effects
B-Lymphocytes / cytology*
CD4 Lymphocyte Count
CD8-Positive T-Lymphocytes / cytology
Cell Division / drug effects
Didanosine / therapeutic use
Female
HIV Infections / drug therapy*,  immunology
HIV Protease Inhibitors / therapeutic use
HIV-1*
Humans
Immunotherapy
Interleukin-2 / therapeutic use*
Lymph Nodes / cytology,  virology
Male
RNA, Viral
Reverse Transcriptase Inhibitors / therapeutic use
Saquinavir / therapeutic use
T-Lymphocytes / cytology*
Zidovudine / therapeutic use
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/HIV Protease Inhibitors; 0/Interleukin-2; 0/RNA, Viral; 0/Reverse Transcriptase Inhibitors; 127779-20-8/Saquinavir; 30516-87-1/Zidovudine; 69655-05-6/Didanosine

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