Document Detail


Lymphocytes as internal standard in oxidative burst analysis by cytometry: A new data analysis approach.
MedLine Citation:
PMID:  22405840     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia.
Authors:
Ilaria Peluso; Giuseppa Morabito; Silvia Riondino; Francesca La Farina; Mauro Serafini
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-3
Journal Detail:
Title:  Journal of immunological methods     Volume:  -     ISSN:  1872-7905     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy.
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