| Lymphocytes as internal standard in oxidative burst analysis by cytometry: A new data analysis approach. | |
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MedLine Citation:
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PMID: 22405840 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We propose a new data analysis approach for reactive oxygen species detection using Dihydrorhodamine123 in blood monocytes and neutrophils. This approach, based on data transformation using lymphocytes as internal standard, allows to appreciate free radical production by monocytes also without Phorbol 12-myristate 13-acetate (PMA) activation. In addition, this method is sensitive to differences in healthy subjects due to sub-pathological conditions, such as hypercholesterolemia. |
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Authors:
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Ilaria Peluso; Giuseppa Morabito; Silvia Riondino; Francesca La Farina; Mauro Serafini |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-3 |
Journal Detail:
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Title: Journal of immunological methods Volume: - ISSN: 1872-7905 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
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Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione (INRAN), Rome, Italy. |
Export Citation:
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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