Document Detail

Lymphocyte predominance Hodgkin's disease: lineage and clonality determination using a single-cell assay.
MedLine Citation:
PMID:  7949084     Owner:  NLM     Status:  MEDLINE    
Lymphocyte predominance Hodgkin's disease (LPHD) is a clinically indolent condition. Although there is evidence that the putative neoplastic cell in this disease, the "L&H" cell, is of B-cell lineage, there is conflicting data concerning the clonality of these cells. Our study was aimed at clarifying the issue of lineage and clonality of the L&H cells of LPHD using a single-cell assay. Four cases of LPHD were studied. To circumvent the difficulties of obtaining fresh tissue and to be able to study representative cases, a new method was developed to obtain single-cell suspensions of L&H cells from archival formalin-fixed paraffin-embedded tissue. Single L&H cells were identified by morphology and immunostaining for epithelial membrane antigen, isolated using a micropipette, and subjected to polymerase chain reaction (PCR) amplification of the complematarity determining region 3 (CDR3) of the Ig heavy chain (IgH) gene, which is B-cell clone-specific. The PCR products were size-fractionated by polyacrylamide gel electrophoresis and representative products were directly sequenced. Single T cells and small B cells were also isolated from the tissues and used as negative and positive controls, respectively. In all four cases of LPHD, the IgH CDR3 of single L&H cells could be amplified. Within each case, the IgH CDR3 of single L&H cells was found to be of different length or of different sequence. Therefore, our results provide strong evidence for the B-cell origin of the L&H cells and the polyclonal nature of LPHD.
J Delabie; A Tierens; G Wu; D D Weisenburger; W C Chan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  84     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1994-12-20     Completed Date:  1994-12-20     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3291-8     Citation Subset:  AIM; IM    
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-3135.
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MeSH Terms
Antigens, CD / analysis
B-Lymphocytes / immunology
Base Sequence
Blotting, Southern
DNA Primers
DNA, Neoplasm / analysis
Hodgkin Disease / genetics,  immunology*,  pathology
Immunoglobulin Heavy Chains / genetics*
Lymphocytes / immunology*,  pathology
Molecular Sequence Data
Polymerase Chain Reaction / methods
Reg. No./Substance:
0/Antigens, CD; 0/DNA Primers; 0/DNA, Neoplasm; 0/Immunoglobulin Heavy Chains

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