Document Detail


Lymphocyte apoptosis in the inflammatory reaction around Taenia solium metacestodes in porcine cysticercosis.
MedLine Citation:
PMID:  16621283     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the current research, we report apoptosis of lymphocytes in the inflammatory reaction around metacestodes in muscle tissue from cysticercotic pigs. Two events, high metacestode viability (100%) and high cysteine protease activity were found to be closely related to a high phosphatydilserine expression by inflammatory lymphocytes (56%). Testing the RPMI medium used for washing away inflammatory cells from metacestodes with 100% viability, with the fluorescent substrate Z-Phe-Ala-AFC for measuring cysteine protease activity, significant fluorescent values were found. In contrast, tests performed with RPMI medium used for washing away inflammatory cells from metacestodes with 90% viability or less, showed low fluorescence values. Flow cytometry analyses of inflammatory cells obtained from four naturally cysticercotic pigs, and stained with Annexin-V/PI, showed lymphocytes expressing phosphatidylserine with values of 0, 6, 41 and 56% on their outer surfaces. Electron microscopy studies of inflammatory cells from metacestodes with 100% viability, showed lymphocytes with strangled and fragmented nuclei, and heterochromatin displaced to the nuclear periphery. In addition, DNA from these cells showed fragmentation in electrophoresis assays. Apoptosis of lymphocytes in the inflammatory reaction around Taenia solium metacestodes, might have been induced by the parasite cysteine protease, and may be involved in impairing cell-mediated immune responses in human and porcine cysticercosis.
Authors:
Sandra Solano; Isabel M Cortés; Natalia I Copitin; Patricia Tato; José L Molinari
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Publication Detail:
Type:  Journal Article     Date:  2006-04-18
Journal Detail:
Title:  Veterinary parasitology     Volume:  140     ISSN:  0304-4017     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-07     Completed Date:  2006-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  171-6     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF 04510, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annexin A5
Apoptosis*
Cells, Cultured
Culture Media
Cysteine Endopeptidases / metabolism
Cysticercosis / immunology,  parasitology,  veterinary*
DNA Fragmentation
Flow Cytometry / veterinary
Fluorescent Dyes / metabolism
Lymphocytes
Muscle, Skeletal / parasitology
Swine
Swine Diseases / immunology*,  parasitology
Taenia solium / enzymology*,  immunology
Chemical
Reg. No./Substance:
0/Annexin A5; 0/Culture Media; 0/Fluorescent Dyes; EC 3.4.22.-/Cysteine Endopeptidases

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