Document Detail

Lymphocyte activation in vivo in the intestinal mucosa of patients with Crohn's disease.
MedLine Citation:
PMID:  3298655     Owner:  NLM     Status:  MEDLINE    
We analyzed lymphocyte activation state in vivo in lamina propria by applying a panel of monoclonal antibodies for cellular activation markers and by identifying different subsets of 3H-thymidine incorporating lymphoblasts by combining autoradiography with immunoperoxidase staining. In focal lymphoblastoid infiltrates 75 +/- 8% of all inflammatory cells displayed Ia, but lymphoid cells expressing 4F2 (40 +/- 6%) and T9 (14 +/- 3%) activation markers were less frequent. The expression of receptors for interleukin-2 (Tac) was particularly low (4 +/- 1%). There were very few 3H-thymidine incorporating cells (labeling index approx. 1%) and most of them belonged to the T cell series. T4+ blasts outnumbered T8+ blasts, and in this numerically minor subpopulation the activated T4/T8 ratio was 2.9 +/- 0.7. Our results suggest that, although a big part of the local inflammatory lymphocytes in Crohn's disease may be activated according to the expression of Ia and 4F2 activation markers, only a fraction of them possess interleukin-2 receptor and have been pushed to the S-phase of the cell cycle.
Y T Konttinen; V Bergroth; D Nordström; M Segerberg-Konttinen; K Seppälä; M Salaspuro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical & laboratory immunology     Volume:  22     ISSN:  0141-2760     ISO Abbreviation:  J Clin Lab Immunol     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-08-11     Completed Date:  1987-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7808987     Medline TA:  J Clin Lab Immunol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  59-63     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal
Crohn Disease / immunology*
Immunoenzyme Techniques
Intestinal Mucosa / immunology*
Lymphocyte Activation*
T-Lymphocytes / classification,  immunology*
Reg. No./Substance:
0/Antibodies, Monoclonal

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