Document Detail


Lymphatic regulation of hematocrit during hypoxia in the toad Bufo woodhousei.
MedLine Citation:
PMID:  7485598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypoxia rapidly increases hematocrit (Hct) in anuran amphibians by reducing plasma volume, but the mechanism(s) mediating this response is unknown. We tested the hypothesis that, during hypoxia, plasma volume is reduced by impaired lymph heart (LH) function, decreasing lymph flow into the circulation. In Bufo woodhousei, we measured the effects of hypoxia on Hct, lymph heart rate (LHR), LH pressure, the movement of dye from the dorsal lymph sac to the arterial blood, and flow through an open LH cannula. We also tested whether splenic contraction or cholinergic nerves contribute to the hypoxia-induced changes. Graded hypoxia between 21 and 4% O2 produced graded increases in Hct (P < 0.0001) and decreases in LHR (P = 0.01). Hypoxia reduced the rate of increase in arterial Evans blue concentration after injection into the dorsal lymph sac (P = 0.041) and decreased flow through an open LH cannula (P < 0.012). Hypoxia increased Hct and reduced LHR similarly in control, splenectomized, and sham-splenectomized toads. Atropine had no significant effect on Hct and LHR. These results indicate that the LHs play a regulatory role in hypoxia-induced hemoconcentration.
Authors:
G M Malvin; S Macías; M Sanchez; R Dasalla; A Park; M Duran
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  269     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-12-12     Completed Date:  1995-12-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R814-21     Citation Subset:  IM    
Affiliation:
Cardiopulmonary Physiology Program, Institute for Basic and Applied Medical Research, Lovelace Institutes, Albuquerque, New Mexico 87108, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / physiopathology*
Arteries
Atropine / pharmacology
Blood / metabolism
Body Fluids / metabolism
Hematocrit*
Lymph / physiology
Lymphatic System / physiopathology*
Pressure
Splenectomy
Grant Support
ID/Acronym/Agency:
HL-07758/HL/NHLBI NIH HHS; HL-38942/HL/NHLBI NIH HHS; RR-03470/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
51-55-8/Atropine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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