| Lymphatic and portal vein absorption of organochlorine compounds in rats. | |
| | |
MedLine Citation:
|
PMID: 19056760 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The route of absorption of ingested compounds is a determinant of their distribution and metabolism. Portal vein absorption results in direct transport to the liver, where metabolism may take place before extrahepatic delivery. Lymphatic absorption can result in delivery of parent compound to nonhepatic tissues. Understanding the fate of an ingested compound requires determination of the importance of each of these routes. Portal vein absorption can be estimated from the difference in concentrations of an ingested compound between the portal vein and peripheral vessel blood. To make these estimations, one must make assumptions on the basis of estimates of flow rate and dilution. We report here methodology that allows a direct measurement of portal vein absorption that is independent of these assumptions. Mesenteric lymph was diverted from rats by cannulation. Portal blood was sampled after duodenal infusion of a bolus of compound of interest along with a portal absorption marker, 3-O-methylglucose. Since lymph was diverted, the appearance in portal blood was solely the result of portal absorption. Absorption was quantified by the areas under the curve for the compound and marker. Portal absorption was a function of the octanol/water partition coefficients for four organochlorine compounds: hexachlorobenzene, pentachlorophenol, DDT, and its metabolite 1,1,1-trichloro-2,2-bischlorophenylethylene. |
| | |
Authors:
|
Ronald J Jandacek; Therese Rider; Qing Yang; Laura A Woollett; Patrick Tso |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-12-04 |
Journal Detail:
|
Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 296 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-01-27 Completed Date: 2009-03-13 Revised Date: 2010-09-23 |
Medline Journal Info:
|
Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: G226-34 Citation Subset: IM |
Affiliation:
|
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45237, USA. Ronald.Jandacek@uc.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3-O-Methylglucose
/
blood Absorption Animals DDT / pharmacokinetics Duodenum / metabolism Hexachlorobenzene / pharmacokinetics Hydrocarbons, Chlorinated / administration & dosage, blood, pharmacokinetics* Indicator Dilution Techniques* Intestinal Absorption Intubation, Gastrointestinal Liver Circulation* Lymph / metabolism* Male Pentachlorophenol / pharmacokinetics Portal Vein / metabolism* Rats Rats, Sprague-Dawley Stomach / metabolism Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
|
ES014464-A1/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Hydrocarbons, Chlorinated; 118-74-1/Hexachlorobenzene; 146-72-5/3-O-Methylglucose; 50-29-3/DDT; 87-86-5/Pentachlorophenol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sodium channel mutation in irritable bowel syndrome: evidence for an ion channelopathy.
Next Document: In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters.