| Lymphatic absorption of fatty acids and cholesterol in the neonatal rat. | |
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MedLine Citation:
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PMID: 10915641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High-fat diets are essential in suckling animals to ensure adequate calories for postnatal growth, but their lymphatic transport of dietary lipids has not been characterized. We established a lymph fistula model in suckling rats to quantify intestinal uptake and lymphatic transport of dietary lipids and analyzed lipoprotein fractions. Suckling 19-day-old Sprague-Dawley rats had their mesenteric lymph ducts cannulated and gastroduodenal tubes inserted. After overnight recovery, [(3)H]triolein and [(14)C]cholesterol were infused for 6 h. Of the total dose, only 38% of triolein and 24% of cholesterol were transported in the lymph of suckling rats. Analyses of residual luminal contents and intestinal mucosal homogenate showed neither reduced absorption nor delayed mucosal processing of ingested lipids to be the cause. Thin-layer chromatographic analysis of radioactive mucosal lipids, however, showed a predominance of free fatty acids (60%) and free cholesterol (67%), implying impaired esterification capacity in these animals. We speculate that this reduced esterification allows for portal transport or direct enterocyte metabolism of dietary lipids. |
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Authors:
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L C Ee; S Zheng; L Yao; P Tso |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 279 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2000 Aug |
Date Detail:
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Created Date: 2000-09-05 Completed Date: 2000-09-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: G325-31 Citation Subset: IM |
Affiliation:
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Division of Pediatric Gastroenterology and Nutrition, Children's Hospital Medical Center, Cincinnati, OH 45267, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Animals, Suckling / metabolism* Apolipoproteins / analysis, metabolism Carbon Radioisotopes / diagnostic use, pharmacokinetics Cholesterol / pharmacokinetics* Chromatography, Thin Layer Chylomicrons / metabolism Dietary Fats / pharmacokinetics Fatty Acids / pharmacokinetics* Female Intestinal Absorption / physiology* Intestinal Mucosa / chemistry, metabolism Lymph / metabolism* Rats Rats, Sprague-Dawley Triolein / pharmacokinetics Tritium / diagnostic use, pharmacokinetics |
| Grant Support | |
ID/Acronym/Agency:
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P01-DK-54504/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/Carbon Radioisotopes; 0/Chylomicrons; 0/Dietary Fats; 0/Fatty Acids; 10028-17-8/Tritium; 122-32-7/Triolein; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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