Document Detail


Lymph node revealing solution: a rapid method for the fixation of cystectomy specimens.
MedLine Citation:
PMID:  10093825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of this study was to describe the use of the lymph node revealing solution (LNRS) for rapid fixation of total cystectomy specimens, and to compare it with formalin fixation. LNRS is a mixture of 95% ethanol, diethyl ether, glacial acetic acid and buffered formalin (65:20:5:10 v/v) prepared under a fume-hood. Sixteen consecutive cystectomy specimens were fixed for two hours either in LNRS or in buffered formalin. Representative sections were embedded in paraffin, sectioned and stained with H&E, periodic acid Schiff, alcian-blue, and immunostained for cytokeratins 20, high and low molecular weight cytokeratins, prostatic specific antigen, Factor VIII related antigen, s-100 protein, and protein kinase C isoenzymes. Results showed that the tissues were well fixed after 2 hours in LNRS, and were not fixed after 2 hours in formalin. Processing and sectioning of the paraffin blocks of the LNRS fixed tissue was excellent; it was impossible in the sections fixed for 2 hours in formalin. All the stains were excellent after LNRS fixation. We conclude that fixation of cystectomy specimens in LNRS requires only two hours and results in excellent stained slides. It is therefore recommended for cystectomy specimens.
Authors:
R Koren; A Paz; M Konichezsky; E Sadikov; B Klein; P Livne; R Gal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pathology, research and practice     Volume:  195     ISSN:  0344-0338     ISO Abbreviation:  Pathol. Res. Pract.     Publication Date:  1999  
Date Detail:
Created Date:  1999-05-18     Completed Date:  1999-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806109     Medline TA:  Pathol Res Pract     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  77-80     Citation Subset:  IM    
Affiliation:
Department of Pathology, Beilinson Hospital, Rabin Medical Center, Petah Tikva, Israel.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Transitional Cell / chemistry,  pathology*,  surgery
Cystectomy*
Fixatives
Histological Techniques
Humans
Immunoenzyme Techniques
Lymph Nodes / pathology*
Staining and Labeling
Tissue Fixation / methods*
Tumor Markers, Biological / analysis
Urinary Bladder Neoplasms / chemistry,  pathology*,  surgery
Chemical
Reg. No./Substance:
0/Fixatives; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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