Document Detail

LuxU connects quorum sensing to biofilm formation in Vibrio fischeri.
MedLine Citation:
PMID:  23035866     Owner:  NLM     Status:  Publisher    
Biofilm formation by Vibrio fischeri is a complex process involving multiple regulators, including the sensor kinase (SK) RscS and the response regulator (RR) SypG, which control the symbiosis polysaccharide (syp) locus. To identify other regulators of biofilm formation in V. fischeri, we screened a transposon library for mutants defective in wrinkled colony formation. We identified LuxQ as a positive regulator of syp-dependent biofilm formation. LuxQ is a member of the Lux phosphorelay and is predicted to control bioluminescence in concert with the SK AinR, the phosphotransferase LuxU and the RR LuxO. Of these, LuxU was the only other regulator that exerted a substantial impact on biofilm formation. We propose a model in which the Lux pathway branches at LuxU to control both bioluminescence and biofilm formation. Furthermore, our evidence suggests that LuxU functions to regulate syp transcription, likely by controlling SypG activity. Finally, we found that, in contrast to its predicted function, the SK AinR has little impact on bioluminescence under our conditions. Thus, this study reveals a novel connection between the Lux and Syp pathways in V. fischeri, and furthers our understanding of how the Lux pathway regulates bioluminescence in this organism.
Valerie A Ray; Karen L Visick
Related Documents :
24462706 - Stratification and delineation of gastric cancer signaling by in vitro transcription fa...
24731196 - Modulation of the sensitivity of jurkat t-cells to inhibition of protein synthesis by t...
6684556 - The effect of n-formylleurosine on dna synthesis of ehrlich ascites tumor.
24802256 - Arachidonic acid induces brain endothelial cell apoptosis via p38-mapk and intracellula...
16934786 - Hyaluronan induces vascular smooth muscle cell migration through rhamm-mediated pi3k-de...
1709096 - Cyclin b targets p34cdc2 for tyrosine phosphorylation.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-5
Journal Detail:
Title:  Molecular microbiology     Volume:  -     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL, 60153, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metabolic syndrome and population attributable risk among HIV/AIDS patients: comparison between NCEP...
Next Document:  The effect of changes in intraocular pressure on the risk of primary open-angle glaucoma in patients...