| Lutein is a competitive inhibitor of cytosolic Ca²+-dependent phospholipase A₂. | |
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MedLine Citation:
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PMID: 21054397 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: We have investigated the effect of lutein on phospholipase A₂ (PLA₂) isozymes. METHODS: We measured arachidonic acid release in [³H]arachidonic acid-labelled Raw 264.7 cells and PLA₂ activity using 1-palmitoyl-2-[¹⁴C]arachidonyl phosphatidylcholine ([¹⁴C]AA-PC) and 10-pyrene phosphatidylcholine in vitro. KEY FINDINGS: Lutein suppressed the release of arachidonic acid and inhibited Raw 264.7 cell-derived cytosolic Ca²+-dependent PLA₂ (cPLA₂-induced hydrolysis of [¹⁴C]AA-PC in a dose- and time-dependent manner. In contrast, lutein did not affect secretory Ca²+-dependent PLA₂ (sPLA₂)-induced hydrolysis of [¹⁴C]AA-PC. A Dixon plot showed that the inhibition by lutein on cPLA₂ appeared to be competitive with an inhibition constant, K(i) , of 13.6 µm. CONCLUSIONS: We suggest that lutein acted as a competitive inhibitor of cPLA₂ but did not affect sPLA₂. |
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Authors:
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Ho Sun Song; Hee Rae Kim; Myung Cheul Kim; Yeon Hee Hwang; Sang Soo Sim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-04 |
Journal Detail:
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Title: The Journal of pharmacy and pharmacology Volume: 62 ISSN: 2042-7158 ISO Abbreviation: J. Pharm. Pharmacol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2011-05-11 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0376363 Medline TA: J Pharm Pharmacol Country: England |
Other Details:
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Languages: eng Pagination: 1711-6 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. JPP © 2010 Royal Pharmaceutical Society of Great Britain. |
Affiliation:
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College of Pharmacy, Chung-Ang University, 221 Huksuk-dong, Dongjak-gu, Seoul 156-756, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology Arachidonic Acid / metabolism Blood Proteins / metabolism, pharmacology* Calcium / metabolism Cells, Cultured Cytosol / enzymology Isoenzymes / antagonists & inhibitors, metabolism Lutein / pharmacology* Mice Phosphatidylcholines / metabolism Phospholipases A2 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents, Non-Steroidal; 0/Blood Proteins; 0/Isoenzymes; 0/PLIalpha; 0/Phosphatidylcholines; 127-40-2/Lutein; 506-32-1/Arachidonic Acid; 7440-70-2/Calcium; EC 3.1.1.4/Phospholipases A2 |
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