| Lung surfactant kinetics in conscious pigs. | |
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MedLine Citation:
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PMID: 10409143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The primary goal of this study was to determine the contributions of plasma free fatty acids (FFA) and de novo synthesized fatty acids (FA) to lung surfactant phosphatidylcholine (PC) synthesis. A new stable isotope tracer model was developed in which [1, 2-(13)C(2)]acetate and uniformly labeled [U-(13)C(16)]palmitate were infused in nine normal overnight fasted pigs to quantify surfactant kinetics in the basal state and during low-dose glucose infusion (2 mg. kg(-1). min(-1)). There was no effect of glucose; therefore, all data were pooled. The surfactant PC-bound palmitate incorporation rate from plasma palmitate was 20.9 +/- 1.9 nmol palmitate. h(-1). g wet lung(-1), compared with the rate of 2.1 +/- 0.3 nmol palmitate. h(-1). g wet lung(-1) from de novo synthesized palmitate. The PC-bound palmitate secretion rate from the lamellar body pool to the alveolar surface pool was 239 +/- 26 nmol palmitate. h(-1). g wet lung(-1). Approximately 90% of the secreted PC recycled back to the lamellar bodies for reutilization. We conclude that plasma is the primary contributor of FA for surfactant PC synthesis under the conditions of this experiment. |
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Authors:
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W Z Martini; D L Chinkes; R E Barrow; E D Murphey; R R Wolfe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 277 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-08-18 Completed Date: 1999-08-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: E187-95 Citation Subset: IM |
Affiliation:
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Shriners Burns Hospital, and Departments of Surgery and Anesthesiology, The University of Texas Medical Branch, Galveston, Texas 77555, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fatty Acids / biosynthesis Fatty Acids, Nonesterified / blood Kinetics Lung / metabolism Palmitates / blood Phosphatidylcholines / metabolism Pulmonary Surfactants / metabolism* Swine |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Palmitates; 0/Phosphatidylcholines; 0/Pulmonary Surfactants |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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