Document Detail


Lung surfactant kinetics in conscious pigs.
MedLine Citation:
PMID:  10409143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The primary goal of this study was to determine the contributions of plasma free fatty acids (FFA) and de novo synthesized fatty acids (FA) to lung surfactant phosphatidylcholine (PC) synthesis. A new stable isotope tracer model was developed in which [1, 2-(13)C(2)]acetate and uniformly labeled [U-(13)C(16)]palmitate were infused in nine normal overnight fasted pigs to quantify surfactant kinetics in the basal state and during low-dose glucose infusion (2 mg. kg(-1). min(-1)). There was no effect of glucose; therefore, all data were pooled. The surfactant PC-bound palmitate incorporation rate from plasma palmitate was 20.9 +/- 1.9 nmol palmitate. h(-1). g wet lung(-1), compared with the rate of 2.1 +/- 0.3 nmol palmitate. h(-1). g wet lung(-1) from de novo synthesized palmitate. The PC-bound palmitate secretion rate from the lamellar body pool to the alveolar surface pool was 239 +/- 26 nmol palmitate. h(-1). g wet lung(-1). Approximately 90% of the secreted PC recycled back to the lamellar bodies for reutilization. We conclude that plasma is the primary contributor of FA for surfactant PC synthesis under the conditions of this experiment.
Authors:
W Z Martini; D L Chinkes; R E Barrow; E D Murphey; R R Wolfe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  277     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-18     Completed Date:  1999-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E187-95     Citation Subset:  IM    
Affiliation:
Shriners Burns Hospital, and Departments of Surgery and Anesthesiology, The University of Texas Medical Branch, Galveston, Texas 77555, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fatty Acids / biosynthesis
Fatty Acids, Nonesterified / blood
Kinetics
Lung / metabolism
Palmitates / blood
Phosphatidylcholines / metabolism
Pulmonary Surfactants / metabolism*
Swine
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Palmitates; 0/Phosphatidylcholines; 0/Pulmonary Surfactants

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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