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Lung remodeling in a porcine model of cyanotic congenital heart defect with decreased pulmonary blood flow.
MedLine Citation:
PMID:  22888850     Owner:  NLM     Status:  In-Data-Review    
ABSTRACT Background: Hypoperfusion of the pulmonary vascular bed under the condition of congenital cardiac malformations may lead to progressive pulmonary vascular disease. To improve the mechanistic understanding of this disease, we examined the biochemical and morphological changes of the lung in a relevant animal model and provided valuable insights into the underlying mechanisms of the pathogenesis of pulmonary hypotension. Materials and Methods: A model of congenital heart defect with decreased pulmonary blood flow was implemented into 8 piglets (the cyanosis group). Another 8 piglets underwent a sham operation (the control group). Two months postoperatively, lung biopsy specimens were harvested for the measurement of the expression levels of MMP-2, MMP-9, TIMP-1, VEGF, and type I and type III collagens. Moreover, the light-microscopic morphology, morphometry, and ultrastructure of lobes were examined. Results: Compared to the controls, the histopathological changes of the pulmonary vasculature in the cyanosis group showed evident hypoplasia and degeneration. The expression levels of MMP-2, MMP-9, TIMP-1, VEGF, and type I collagen, as well as the microvessel density, in the cyanosis group were significantly lower than those in the control group, whereas the level of type III collagen in the cyanosis group was significantly higher than that in the control group. Conclusions: The observed morphological changes may represent an adaptive reaction to the prolonged decrease of pulmonary blood flow. The underlying mechanism of lung remodeling may be attributed to the changes in the expression of structural proteins and cytokines in the pulmonary extracellular matrix induced by modulating factors.
Yaoqiang Xu; Yinglong Liu; Zhiqiang Li; Junwu Su; Gang Li; Lizhong Sun
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental lung research     Volume:  38     ISSN:  1521-0499     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  355-62     Citation Subset:  IM    
Pediatric Cardiac Center, Beijing Anzhen Hospital, The Capital Medical University, Beijing, China.
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