| Lung preservation: pulmonary flush route affects bronchial mucosal temperature and expression of IFN-gamma and Gro in regional lymph nodes. | |
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MedLine Citation:
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PMID: 15816879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Optimal lung preservation via flush of the pulmonary vasculature minimizes early graft failure post-lung transplantation. We hypothesized that the route of pulmonary flush has differential effects on thermal gradients in the lung and expression of inflammatory mediators. Swine underwent antegrade flush (AG) via pulmonary artery; AG/RG: antegrade + retrograde flush via pulmonary veins or AG/BA: antegrade + bronchial artery flush via bronchial artery. Temperatures were recorded in bronchial mucosa and peribronchial lymph nodes. RT-PCR was utilized to detect cytokine gene expression in the nodes. AG/BA flush resulted in greatest cooling of bronchial mucosa and lymph nodes (p < 0.001). The route of flush did not affect expression of RANTES, MCP-1, IL-8, IL-1beta, TNF-alpha or IL-6. However, expression of Gro was reduced 4-h post-preservation in all groups. Only AG/BA resulted in decreased IFN-gamma transcripts. These data show that, compared to AG or AG/RG, AG/BA flush results in the greatest cooling of lung compartments and down regulates lymph node expression of a cytokine and chemokine that have key roles in inflammation and immunity. These data suggest that pulmonary flush via AG/BA during donor harvest may be optimal to decrease the risk of early graft failure. |
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Authors:
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David W Roe; John W Fehrenbacher; Michael R Niemeier; Michael Zieger; Carlos Labarrere; David S Wilkes |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons Volume: 5 ISSN: 1600-6135 ISO Abbreviation: Am. J. Transplant. Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-04-08 Completed Date: 2005-08-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100968638 Medline TA: Am J Transplant Country: Denmark |
Other Details:
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Languages: eng Pagination: 995-1001 Citation Subset: IM |
Affiliation:
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Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Temperature Bronchi / pathology Chemokine CCL2 / biosynthesis Chemokine CCL5 / biosynthesis DNA Primers / chemistry Female Gene Expression Regulation Graft Survival Interferon-gamma / biosynthesis, metabolism Interleukin-1 / biosynthesis Interleukin-6 / biosynthesis Interleukin-8 / biosynthesis Lung / metabolism, pathology* Lung Transplantation / methods* Lymph Nodes / pathology Male Mucous Membrane / metabolism, pathology* Organ Preservation / methods* Organ Preservation Solutions Preservation, Biological / methods* Pulmonary Artery / pathology Random Allocation Reverse Transcriptase Polymerase Chain Reaction Swine Temperature Time Factors Tumor Necrosis Factor-alpha / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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HL67177/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Chemokine CCL5; 0/DNA Primers; 0/Interleukin-1; 0/Interleukin-6; 0/Interleukin-8; 0/Organ Preservation Solutions; 0/Tumor Necrosis Factor-alpha; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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