Document Detail


Lung microvascular endothelium is enriched with progenitor cells that exhibit vasculogenic capacity.
MedLine Citation:
PMID:  18065657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endothelial progenitor cells (EPCs) have been isolated postnatally from bone marrow, blood, and both the intima and adventitia of conduit vessels. However, it is unknown whether EPCs can be isolated from the lung microcirculation. Thus we sought to determine whether the microvasculature possesses EPCs capable of de novo vasculogenesis. Rat pulmonary artery (PAEC) and microvascular (PMVEC) endothelial cells were isolated and selected by using a single-cell clonogenic assay. Whereas the majority of PAECs (approximately 60%) were fully differentiated, the majority of PMVECs (approximately 75%) divided, with approximately 50% of the single cells giving rise to large colonies (>2,000 cells/colony). These highly proliferative cells exhibited the capacity to reconstitute the entire proliferative hierarchy of PMVECs, unveiling the existence of resident microvascular endothelial progenitor cells (RMEPCs). RMEPCs expressed endothelial cell markers (CD31, CD144, endothelial nitric oxide synthase, and von Willenbrand factor) and progenitor cell antigens (CD34 and CD309) but did not express the leukocyte marker CD45. Consistent with their origin, RMEPCs interacted with Griffonia simplicifolia and displayed restrictive barrier properties. In vitro and in vivo Matrigel assays revealed that RMEPCs possess vasculogenic capacity, forming ultrastructurally normal de novo vessels. Thus the pulmonary microcirculation is enriched with EPCs that display vasculogenic competence while maintaining functional endothelial microvascular specificity.
Authors:
Diego F Alvarez; Lan Huang; Judy A King; M Khair ElZarrad; Mervin C Yoder; Troy Stevens
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-12-07
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  294     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-10     Completed Date:  2008-05-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L419-30     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Center for Lung Biology, College of Medicine, University of South Alabama, Mobile, AL 36688, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD / analysis
Antigens, CD31 / analysis
Antigens, CD34 / analysis
Antigens, CD45 / analysis
Cadherins / analysis
Cell Proliferation
Endothelium, Vascular / cytology*
Glycoproteins / analysis
Lung / blood supply*
Microcirculation / cytology*
Neovascularization, Physiologic / physiology*
Nitric Oxide Synthase Type II / analysis
Nitric Oxide Synthase Type III
Peptides / analysis
Phenotype
Rats
Stem Cells / physiology*
Telomere / metabolism
Vascular Endothelial Growth Factor Receptor-2 / analysis
Grant Support
ID/Acronym/Agency:
HL-60024/HL/NHLBI NIH HHS; HL-66299/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Antigens, CD31; 0/Antigens, CD34; 0/Cadherins; 0/Glycoproteins; 0/Peptides; 0/cadherin 5; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2; EC 3.1.3.48/Antigens, CD45
Comments/Corrections
Comment In:
Am J Physiol Lung Cell Mol Physiol. 2008 Mar;294(3):L417-8   [PMID:  18192593 ]

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