Document Detail


Lung lining fluid antioxidants in male broilers: age-related changes under thermoneutral and cold temperature conditions.
MedLine Citation:
PMID:  9872595     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine age-related changes in lung lining fluid antioxidants in broilers reared under thermoneutral or cold temperature conditions. Male broilers (Cobb 500) were placed in floor pens within environmental chambers and fed a standard commercial starter diet. The thermoneutral Control chamber was maintained at 32, 30, 27, and 22 to 25 C for Weeks 1, 2, 3, and 4 to 7, respectively, whereas temperature in the Cold chamber was lowered to 18 C during Week 3 and maintained between 15 and 18 C for the rest of the study. At 2, 4, and 7 wk, four to six birds per chamber were selected randomly. The lungs were lavaged with heparinized saline (2 mL/g lung) to obtain lung lining fluid. Antioxidants [reduced (GSH), oxidized (GSSG), and total (TGSH) glutathione, uric acid, ascorbic acid, and alpha- and gamma-tocopherol] in lung lining fluid were determined by HPLC; protein was determined colorimetrically. In Controls, levels of alpha- and -gamma-tocopherol, uric acid, and GSH in lung lining fluid decreased between 2 and 7 wk of age. Birds in the Cold chamber exhibited higher protein, a higher GSSG:TGSH ratio, and a decrease in ascorbic acid (7 wk) in lung lining fluid relative to Controls. Lung lining fluid antioxidants were not correlated with antioxidants in plasma. To determine the effect of vitamin E supplementation on lung lining fluid antioxidants, birds were given a supplement of 200 IU alpha-tocopherol per day for 7 d. Alpha-tocopherol supplementation elevated alpha-tocopherol levels in lung lining fluid, but lowered ascorbic acid, GSH, and GSSG and had no effect on uric acid in lung lining fluid. The results of this study suggest that antioxidant protection in lung lining fluid may diminish with age, that cold conditions in this study produced an oxidative stress in lung lining fluid in broilers, and that oral supplementation of alpha-tocopherol elevated lung lining fluid alpha-tocopherol.
Authors:
W G Bottje; S Wang; K W Beers; D Cawthon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Poultry science     Volume:  77     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-25     Completed Date:  1999-02-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1905-12     Citation Subset:  IM    
Affiliation:
Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville 72701, USA. wbottje@comp.uark.edu
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MeSH Terms
Descriptor/Qualifier:
Aging*
Animals
Antioxidants / metabolism*
Ascorbic Acid / metabolism
Body Fluids / metabolism*
Chickens / metabolism*
Chromatography, High Pressure Liquid
Cold Temperature*
Glutathione / blood,  metabolism
Irrigation
Lung / metabolism*
Male
Oxidation-Reduction
Uric Acid / blood,  metabolism
Vitamin E / administration & dosage,  blood,  metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 69-93-2/Uric Acid; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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