| Lung inflammation and pulmonary function in infants with meconium aspiration syndrome. | |
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MedLine Citation:
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PMID: 17722052 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the relationship between inflammation and pulmonary function, we quantified changes in inflammatory cellular profile, pro-inflammatory cytokines, and pulmonary function in intubated neonates with meconium aspiration syndrome (MAS). METHODS: Sixteen term infants were studied. Tracheal aspirate fluids, obtained within the first 6, 24, 48, and 96 hr of life were used for measurements of: (1) cellular profile changes; (2) mRNA and protein levels for pro-inflammatory cytokines, IL-1beta, IL-6, IL-8, and TNF-alpha, using RT-PCR and ELISA. Using the same time points as above, we determined mean airway pressure, oxygenation index (OI), alveolar-arterial oxygen gradient, and arterial/alveolar oxygen ratio. Baseline tidal volume and pulmonary compliance were obtained. RESULTS: Birth weight was 3,820 +/- 656 g, gestational age 39.8 +/- 1.4 weeks. Mean airway pressure and OI significantly decreased from the first 6-96 hr of age (P = 0.01, P = 0.027). Cell counts were elevated in the first 6 hr compared to 96 hr (17.4 x 10(6)/ml vs. 1.5 x 10(6)/ml, P < 0.05). Pro-inflammatory cytokines decreased from the first 6-96 hr: IL-1beta (187 vs. 37 pg/ml, P < 0.05); IL-6 (3,469 vs. 150 pg/ml, P < 0.05); IL-8 (16,230 vs. 6,334 pg/ml, P = 0.01). CONCLUSIONS: MAS is associated with an inflammatory response characterized by the presence of elevated cell count and pro-inflammatory cytokines which significantly decreased by 96 hr of life. This decrease in lung inflammation has a positive correlation with corresponding decreases in mean airway pressure and oxygenation index, two parameters associated with improved pulmonary function. |
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Authors:
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Rowena G Cayabyab; Kenny Kwong; Craig Jones; Parviz Minoo; Manuel Durand |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Pediatric pulmonology Volume: 42 ISSN: 8755-6863 ISO Abbreviation: Pediatr. Pulmonol. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-04 Completed Date: 2008-02-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510590 Medline TA: Pediatr Pulmonol Country: United States |
Other Details:
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Languages: eng Pagination: 898-905 Citation Subset: IM |
Copyright Information:
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(c) 2007 Wiley-Liss, Inc. |
Affiliation:
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Division of Neonatal Medicine, Department of Pediatrics, LAC+USC Medical Center, University of Southern California, Keck School of Medicine, Los Angeles, California, 90033, USA. cayabyab@usc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytokines
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biosynthesis* Humans Infant, Newborn Inflammation Lung / immunology Lymphocyte Count* Meconium Aspiration Syndrome / immunology*, physiopathology RNA, Messenger / analysis Respiration / immunology Respiratory Function Tests Reverse Transcriptase Polymerase Chain Reaction Trachea |
| Grant Support | |
ID/Acronym/Agency:
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HL60231/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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