Document Detail


Lung function, permeability, and surfactant composition in oleic acid-induced acute lung injury in rats.
MedLine Citation:
PMID:  11076799     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although acute lung injury (ALI) is associated with inflammation and surfactant dysfunction, the precise sequence of these changes remains poorly described. We used oleic acid to study the pathogenesis of ALI in spontaneously breathing anesthetized rats. We found that lung pathology can occur far more rapidly than previously appreciated. Lung neutrophils were increased approximately threefold within 5 min, and surfactant composition was dramatically altered within 15 min. Alveolar cholesterol increased by approximately 200%, and even though disaturated phospholipids increased by approximately 30% over 4 h, the disaturated phospholipid-to-total phospholipid ratio fell. Although the alveolocapillary barrier was profoundly disrupted after just 15 min, with marked elevations in lung fluid ((99m)Tc-labeled diethylenetriamine pentaacetic acid) and (125)I-labeled albumin flux, the lung rapidly began to regain its sieving properties. Despite the restoration in lung permeability, the animals remained hypoxic even though minute ventilation was increased approximately twofold and static compliance progressively deteriorated. This study highlights that ALI can set in motion a sequence of events continuing the respiratory failure irrespective of the alveolar surfactant pool size and the status of the alveolocapillary barrier.
Authors:
K G Davidson; A D Bersten; H A Barr; K D Dowling; T E Nicholas; I R Doyle
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  279     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2000-12-01     Completed Date:  2000-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  L1091-102     Citation Subset:  IM    
Affiliation:
Department of Human Physiology, School of Medicine, Flinders University of South Australia, Australia.
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MeSH Terms
Descriptor/Qualifier:
Albumins / pharmacokinetics
Animals
Blood Gas Analysis
Body Fluid Compartments / physiology
Bronchoalveolar Lavage Fluid / chemistry,  cytology
Capillary Permeability / physiology
Iodine Radioisotopes / diagnostic use
Leukocyte Count
Lung Compliance / physiology*
Lysophosphatidylcholines / metabolism
Macrophages, Alveolar / cytology
Male
Oleic Acid*
Organ Size
Pneumonia / chemically induced,  pathology,  physiopathology
Pulmonary Alveoli / metabolism,  physiopathology*
Rats
Rats, Inbred Strains
Respiratory Distress Syndrome, Adult / chemically induced,  pathology,  physiopathology*
Surface-Active Agents / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Albumins; 0/Iodine Radioisotopes; 0/Lysophosphatidylcholines; 0/Surface-Active Agents; 112-80-1/Oleic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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